{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T22:05:42Z","timestamp":1773785142584,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T00:00:00Z","timestamp":1549497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To study oral <jats:italic>Prevotella<\/jats:italic> spp. isolated from patients with chronic periodontitis, to determine their susceptibility to azithromycin and erythromycin and to screen the presence of macrolide resistance genes therein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Isolates with a <jats:italic>Prevotella<\/jats:italic>\u2010like morphology were obtained from subgingival samples of 52 patients with chronic periodontitis. Each isolate was identified to the species level by sequencing of the 16S rRNA gene. In 100 <jats:italic>Prevotella<\/jats:italic> spp. isolates, azithromycin and erythromycin susceptibility was determined using the <jats:italic>E<\/jats:italic> test method, and the screening of <jats:italic>erm<\/jats:italic>(A), <jats:italic>erm<\/jats:italic>(B), <jats:italic>erm<\/jats:italic>(C), <jats:italic>erm<\/jats:italic>(F), <jats:italic>erm<\/jats:italic>(G), <jats:italic>erm<\/jats:italic>(Q) and <jats:italic>mef<\/jats:italic>(A) genes was done by PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Prevotella intermedia <\/jats:italic>and <jats:italic>Prevotella nigrescens<\/jats:italic> were the most identified species (33% each). Minimum inhibitory concentrations (MICs) ranges for both antibiotics were 0.016\/0.032 to &gt;256\u00a0\u03bcg\/ml. MIC<jats:sub>50<\/jats:sub> values for azithromycin and erythromycin were 1.5 and 1\u00a0\u03bcg\/ml, respectively, and MIC<jats:sub>90<\/jats:sub> values were &gt;256\u00a0\u03bcg\/ml for both antibiotics. Nineteen per cent of the isolates carried <jats:italic>erm<\/jats:italic>(B), and 51% carried <jats:italic>erm<\/jats:italic>(F).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The MIC values found were high compared to previous studies. <jats:italic>erm<\/jats:italic>(F) was greatly prevalent, and we describe for the first time the <jats:italic>erm<\/jats:italic>(B) gene in <jats:italic>Prevotella <\/jats:italic>spp. The presence of either of the genes seems to be associated with a higher degree of resistance to azithromycin and erythromycin.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13043","type":"journal-article","created":{"date-parts":[[2019,1,23]],"date-time":"2019-01-23T20:41:54Z","timestamp":1548276114000},"page":"860-867","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Azithromycin and erythromycin susceptibility and macrolide resistance genes in <i>Prevotella <\/i>from patients with periodontal disease"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4459-1300","authenticated-orcid":false,"given":"Alexandre","family":"Arredondo","sequence":"first","affiliation":[{"name":"Department of Microbiology Dentaid Research Center Cerdanyola del Vall\u00e8s Spain"},{"name":"Departament de Gen\u00e8tica i Microbiologia Universitat Aut\u00f2noma de Barcelona Bellaterra Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Blanc","sequence":"additional","affiliation":[{"name":"Department of Microbiology Dentaid Research Center Cerdanyola del Vall\u00e8s Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Mor","sequence":"additional","affiliation":[{"name":"Department of Periodontology Universitat Internacional de Catalunya Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Nart","sequence":"additional","affiliation":[{"name":"Department of Periodontology Universitat Internacional de Catalunya Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rub\u00e9n","family":"Le\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Microbiology Dentaid Research Center Cerdanyola del Vall\u00e8s Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5721-5732.2005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.4.592"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx290"},{"key":"e_1_2_8_6_1","first-page":"1","article-title":"The European community strategy against antimicrobial resistance","volume":"9","author":"Bronzwaer S.","year":"2004","journal-title":"European Surveillance"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2002.290210.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.1.5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.345-348.2001"},{"key":"e_1_2_8_10_1","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"CLSI","year":"2017"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1301970"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-014-1249-7"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2017.4872"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12075"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2008.01260.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2011.01418.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110558"},{"key":"e_1_2_8_18_1","unstructured":"Kaplan W. 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