{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:49:31Z","timestamp":1747187371461,"version":"3.40.5"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T00:00:00Z","timestamp":1552867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600836"],"award-info":[{"award-number":["81600836"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016M591718"],"award-info":[{"award-number":["2016M591718"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the alternative spliced isoforms of Fibronectin (FN) in the stroma of radicular cysts and analyze the associations between these isoforms and the osteoclastogenic effects of fibroblasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>The specimens of radicular cysts were stained with immunohistochemistry, and the associations between each FN isoform and clinical parameters were assessed. The fibroblasts isolated from cysts or jaw bone were cultured to induce the Trap\u00a0+\u00a0MNCs. In the conditioned medium, the Fibronectin containing extra domain A (EDA\u00a0+\u00a0FN) was neutralized by antibody IST\u20109, and the EDA exon of fibroblasts was knockout by CRISPR\/Cas system, for assessing the osteoclastogenic effects. The mRNA level of FN isoforms and the osteoclastogenesis\u2010related genes were analyzed by quantitive PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EDA\u00a0+\u00a0FN staining was positively associated with the size of the lesions (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). In contrast with the controls, the ratio of EDA\u00a0+\u00a0FN\/total FN in the fibroblasts from radicular cysts was significantly higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), and positively associate with Trap\u00a0+\u00a0MNCs counting, it was consistent with increased expression of COX\u20102, IL\u20106, IL\u201017, and the RANKL\/OPG (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The Trap\u00a0+\u00a0MNCs counting and osteoclastogenesis\u2010related genes were decreased by IST\u20109 blocking and EDA exon knockout in fibroblasts, but the blockage of the interaction between EDA\u00a0+\u00a0FN and pre\u2010osteoclasts exhibited little effects on Trap\u00a0+\u00a0MNCs formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The microenvironment of the fibrous capsule of radicular cysts facilitates the splicing of EDA exon, it endues EDA\u00a0+\u00a0FN with autocrine effects on fibroblast itself, and it increases the expression of osteoclastogenesis\u2010related genes, by which the osteoclastogenesis in radicular cysts could be initiated.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13064","type":"journal-article","created":{"date-parts":[[2019,2,16]],"date-time":"2019-02-16T08:03:20Z","timestamp":1550304200000},"page":"1136-1146","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The fibroblast of radicular cyst facilitate osteoclastogenesis via the autocrine of Fibronectin containing extra domain A"],"prefix":"10.1111","volume":"25","author":[{"given":"Chun\u2010yan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Endodontics, School &amp; Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration Tongji University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8189-6424","authenticated-orcid":false,"given":"Hai\u2010Cheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, School &amp; Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration Tongji University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2015.08.036"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.2000.1610"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1259\/dmfr\/29360754"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2011.05.009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00212.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116975"},{"key":"e_1_2_8_8_1","first-page":"E585","article-title":"The post\u2010endodontic periapical lesion: Histologic and etiopathogenic aspects","volume":"12","author":"Garcia C. C.","year":"2007","journal-title":"Medicina Oral, Patologia Oral Y Cirugia Bucal"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1206730"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/adj.12174"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02890411"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijos.2013.96"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jicd.12044"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2013.01.016"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-330"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-005-9017-6"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-154435"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2004.02.032"},{"key":"e_1_2_8_19_1","first-page":"1059","article-title":"Analysis of Soluble molecular fibronectin\u2010fibrin complexes and EDA\u2010Fibronectin concentration in plasma of patients with atherosclerosis","volume":"39","author":"Lemanska\u2010Perek A.","year":"2016","journal-title":"Inflammation"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-015-0377-6"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.21136"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.05.007"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2003.00379.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-011-0271-1"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700653"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12231"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2012.01966.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12323"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.06.028"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201210152"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510379019"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.7489"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fodi.13064","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.13064","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/odi.13064","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.13064","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T04:55:07Z","timestamp":1694148907000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.13064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,18]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1111\/odi.13064"],"URL":"https:\/\/doi.org\/10.1111\/odi.13064","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"type":"print","value":"1354-523X"},{"type":"electronic","value":"1601-0825"}],"subject":[],"published":{"date-parts":[[2019,3,18]]},"assertion":[{"value":"2018-11-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}