{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:55:54Z","timestamp":1785855354573,"version":"3.56.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T00:00:00Z","timestamp":1555027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE020147"],"award-info":[{"award-number":["DE020147"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine expression and localization of membrane\u2010associated mucins within human keratinized and non\u2010keratinized oral epithelia, and to explore transcriptional changes associated with primary Sj\u00f6gren's syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Mucin transcripts and glycoproteins were determined by RT\u2010PCR and immunohistochemistry, respectively, in oral keratinized (hard palate) and non\u2010keratinized (buccal) epithelia obtained from three cadavers. Mucin transcripts assessed by quantitative PCR were compared between cells harvested by brushing buccal and palatal epithelia of 25 female primary Sj\u00f6gren's syndrome patients vs 25 healthy age\u2010matched female control subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In hard palate, MUC4 is absent and MUC1 localized to deeper cell layers. Both mucins are within the apical layers of buccal epithelium. MUC15 is localized throughout all palatal cell layers and in all but the basal layer of buccal epithelia. MUC16, MUC20, and MUC21 glycoproteins are localized within all but the basal cell layer of both tissue types. In buccal cells of primary Sj\u00f6gren's patients, MUC21 transcripts are down\u2010regulated 3.4\u2010fold and MUC20 2.6\u2010fold. Dysregulation of select epithelial mucins may therefore contribute to xerostomia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Differential expression of multiple mucins and down\u2010regulation in Sj\u00f6gren's syndrome support further study of oral epithelial mucin physiology and pathophysiology, including their functions in hydration and lubrication of the oral mucosal pellicle.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13098","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T13:10:56Z","timestamp":1553778656000},"page":"1325-1334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Oral epithelial membrane\u2010associated mucins and transcriptional changes with Sj\u00f6gren's syndrome"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2124-6347","authenticated-orcid":false,"given":"David J.","family":"Culp","sequence":"first","affiliation":[{"name":"Department of Oral Biology, College of Dentistry University of Florida Gainesville Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3781-6308","authenticated-orcid":false,"given":"Carol","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Oral &amp; Maxillofacial Diagnostic Sciences, College of Dentistry University of Florida Gainesville Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4650-651X","authenticated-orcid":false,"given":"Shannon M.","family":"Wallet","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, College of Dentistry University of Florida Gainesville Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2007.078246"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2840557"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459988910100317"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.10.018"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.052993"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cocis.2010.07.002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.05.003"},{"issue":"7","key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.4321\/S0365-66912003000700006","article-title":"Normal human conjunctival epithelium expresses MUC13, MUC15, MUC16 and MUC17 mucin genes","volume":"78","author":"Corrales R. 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