{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:29:06Z","timestamp":1762507746163,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T00:00:00Z","timestamp":1560729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008549","name":"Shanxi Medical University","doi-asserted-by":"publisher","award":["057708","032237"],"award-info":[{"award-number":["057708","032237"]}],"id":[{"id":"10.13039\/501100008549","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify immune\u2010related gene expression profiles of adipocytes under low temperatures with RNA sequencing as a model for Bell's palsy implications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adipocytes were harvested from the white adipose tissue of male Sprague\u2010Dawley rats and cultured under different acute\u2010grade cold exposure conditions of 30, 20, and 10\u00b0C, and their genomes were sequenced for RNA sequencing analysis. The differentially expressed genes (DEGs) were validated with reverse transcription polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 55 (35 upregulated and 20 downregulated), 121 (76 upregulated and 45 downregulated), and 92 (64 upregulated and 28 downregulated) DEGs were identified under 30, 20, and 10\u00b0C compared with the control, respectively. KEGG and GO analysis revealed that the DEGs were considerably enriched in immune\u2010related pathways (leukocyte transendothelial migration and platelet activation) and infection (bacterial invasion of epithelial cells and <jats:italic>Salmonella<\/jats:italic> infection). The levels of key inflammatory chemokines (<jats:italic>CSF1<\/jats:italic>, <jats:italic>CXCL1<\/jats:italic>, <jats:italic>CCL2<\/jats:italic>, and <jats:italic>CCL7<\/jats:italic>) were enhanced after cold exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings broaden our understanding of the immune responses to cold exposure in adipocytes. The molecular profiles of adipocyte immune function will help clarify the potential mechanism impacting myelin, which might contribute to the development of strategies to control Bell's palsy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13126","type":"journal-article","created":{"date-parts":[[2019,5,25]],"date-time":"2019-05-25T14:09:22Z","timestamp":1558793362000},"page":"1652-1663","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immune\u2010related gene expression profiles of hypothermia adipocytes: Implications for Bell's palsy"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1728-3636","authenticated-orcid":false,"given":"Wenjuan","family":"Zhang","sequence":"first","affiliation":[{"name":"School and Hospital of Stomatology Shanxi Medical University Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Xu","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology Shanxi Medical University Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Luo","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology Shanxi Medical University Taiyuan China"},{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology &amp; Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Zhao","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology Shanxi Medical University Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Wu","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology Shanxi Medical University Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianqi","family":"Li","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology Shanxi Medical University Taiyuan China"},{"name":"Department of Oral and Maxillofacial Surgery Matsumoto Dental University Shiojiri Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(75)90109-4"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.12691\/ijdsr-2-5A-1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.2.301"},{"key":"e_1_2_10_5_1","first-page":"315","article-title":"Pathophysiology of subcutaneous fat","volume":"148","author":"Cassisa A.","year":"2013","journal-title":"Giornale Italiano Dermatologia e Venereology"},{"key":"e_1_2_10_6_1","first-page":"1","article-title":"Adipose tissue expression of PACAP, VIP, and their receptors in response to cold stress","author":"Cline D. L.","year":"2018","journal-title":"Journal of Molecular Neuroscience"},{"key":"e_1_2_10_7_1","first-page":"700","article-title":"The clinical significance of the neutrophil\u2010to\u2010lymphocyte ratio in multiple sclerosis","volume":"126","author":"Demirci S.","year":"2016","journal-title":"International Journal of Neuroscience"},{"key":"e_1_2_10_8_1","first-page":"99","article-title":"An experimental study of the culture, isolation and biological characteristics of rat adipose tissue\u2010derived stromal cells in vitro","volume":"14","author":"Deng C. H.","year":"2008","journal-title":"Zhonghua Nan Ke Xue"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2015.10.047"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2014-309563"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000492671"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.05.008"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118732748.ch8"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22796"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/00207454.2013.763804"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2014.0074"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2012.10.008"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.2016.1190121"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2015.11.033"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000433542"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599814555841"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1515\/pjvs-2016-0056"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874364101812010041"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.94716"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17050795"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.10.069"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2014.03.001"},{"key":"e_1_2_10_28_1","first-page":"10875","article-title":"Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway in vitro","volume":"8","author":"Nie Y.","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162512"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1590\/0004-282X20160132"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00473.2013"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000092779"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2013.04.002"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2002.8035"},{"key":"e_1_2_10_35_1","first-page":"399","article-title":"Difference of the adipose forms and functions in rat's different parts","volume":"34","author":"Wang C.","year":"2015","journal-title":"Journal of Southeast University"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21386"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2018.04.007"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1159\/000455073"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.13126","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/odi.13126","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.13126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T17:14:08Z","timestamp":1694279648000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.13126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,17]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1111\/odi.13126"],"URL":"https:\/\/doi.org\/10.1111\/odi.13126","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"type":"print","value":"1354-523X"},{"type":"electronic","value":"1601-0825"}],"subject":[],"published":{"date-parts":[[2019,6,17]]},"assertion":[{"value":"2019-01-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}