{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:29:22Z","timestamp":1790900962024,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008549","name":"Shanxi Medical University","doi-asserted-by":"publisher","award":["KY201803"],"award-info":[{"award-number":["KY201803"]}],"id":[{"id":"10.13039\/501100008549","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008549","name":"Shanxi Medical University","doi-asserted-by":"publisher","award":["03201430"],"award-info":[{"award-number":["03201430"]}],"id":[{"id":"10.13039\/501100008549","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81271144"],"award-info":[{"award-number":["81271144"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>There are challenges in the treatment of chronic inflammatory diseases of oral mucosa. Both paeoniflorin (PF) and baicalin (BAI) exert anti\u2010inflammatory effects, but the mechanism underlying their combined effects is still unclear. Here, we explored the anti\u2010inflammatory function of the PF\u2010BAI combination in the oral inflammatory response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The CCK\u20108 assay was used to determine the proliferative capacity of HOKs with PF and BAI. Enzyme\u2010linked immunosorbent (ELISA), Western blotting, reverse transcription polymerase chain reaction, and confocal immunofluorescence were performed to study the anti\u2010inflammatory effects of PF\u2010BAI in LPS\u2010stimulated human oral keratinocytes (HOKs). Immunohistochemistry and ELISA were performed to detect the levels of NF\u2010\u03baB p65, IKK\u03b1 and IL\u20106, TNF\u2010\u03b1 in OLP and healthy tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to PF or BAI alone, the combination of PF\u2010BAI at 5\u00a0\u00b5g\/ml downregulated secretion of inflammatory cytokines more effectively (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Combined PF\u2010BAI decreased NF\u2010\u03baB p65 and I\u03baB\u03b1 protein phosphorylation, leading to reduce nuclear translocation of NF\u2010\u03baB p65. Higher expression of TNF\u2010\u03b1, IL\u20106, NF\u2010\u03baB p65, and IKK\u03b1 were observed in OLP than in HC tissues (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The optimal combination concentration of PF and BAI at 5\u00a0\u00b5g\/ml may have a positive effect on the treatment of oral inflammatory diseases, providing a novel therapeutic approach.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13171","type":"journal-article","created":{"date-parts":[[2019,8,8]],"date-time":"2019-08-08T14:43:25Z","timestamp":1565275405000},"page":"1945-1953","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Anti\u2010inflammatory effect of paeoniflorin combined with baicalin in oral inflammatory diseases"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3692-3408","authenticated-orcid":false,"given":"Feiyan","family":"Yu","sequence":"first","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoyin","family":"Li","sequence":"additional","affiliation":[{"name":"Jilin University School and Hospital of Stomatology Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Li","sequence":"additional","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejun","family":"Ge","sequence":"additional","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6527-1165","authenticated-orcid":false,"given":"Fang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyun","family":"Ren","sequence":"additional","affiliation":[{"name":"Shanxi Medical University School and Hospital of Stomatology Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.04.042"},{"key":"e_1_2_7_3_1","first-page":"10139","article-title":"Baicalin ameliorates isoproterenol\u2010induced acute myocardial infarction through iNOS, inflammation and oxidative stress in rat","volume":"8","author":"Chen H. 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