{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T23:47:20Z","timestamp":1768348040092,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T00:00:00Z","timestamp":1575331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing","doi-asserted-by":"publisher","award":["cstc2016jcyjA0243"],"award-info":[{"award-number":["cstc2016jcyjA0243"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003075","name":"Chongqing Municipal Public Health Bureau, Chongqing People's Municipal Government","doi-asserted-by":"publisher","award":["2016MSXM049"],"award-info":[{"award-number":["2016MSXM049"]}],"id":[{"id":"10.13039\/501100003075","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Rat dental follicle cells (rDFCs) function as precursor cells of periodontal tissues. Bone morphogenetic protein (BMP9) plays an important role in proliferation and differentiation. Tumour necrosis factor\u2010alpha (TNF\u2010alpha) is an important contributor to bone resorption. Wnt canonical pathway can be inhibited by Dickkopf 1 (DKK1). The aim of the study was to enhance the osteogenesis of BMP9 treated rDFCs in an inflammatory environment and elucidate the mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>rDFCs were infected by adenoviruses expressing BMP9 (adBMP9). Expression levels of proteins and genes were measured by Western blotting and qPCR. The effect on osteogenesis was evaluated by measuring the activity of alkaline phosphatase (ALP), observation of Alizarin Red S and haematoxylin and eosin staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TNF\u2010alpha activated the canonical Wnt pathway and inhibited the non\u2010canonical pathway. DKK1 suppressed the canonical pathway and promoted the non\u2010canonical pathway. Addition of TNF\u2010alpha or DKK1 inhibited BMP9\/Smad pathway. However, this inhibition was reduced by the addition of DKK1 with TNF\u2010alpha.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>DKK1 reduces the inhibitory effects of TNF\u2010alpha in adBMP9\u2010infected\u2010rDFCs, possibly through interaction with the Smad signalling pathway and Wnt pathways. These findings may lead to a novel approach for the treatment of periodontitis\u2010related alveolar bone defects.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13235","type":"journal-article","created":{"date-parts":[[2019,11,16]],"date-time":"2019-11-16T21:04:22Z","timestamp":1573938262000},"page":"360-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["DKK1 and TNF\u2010alpha influence osteogenic differentiation of adBMP9\u2010infected\u2010rDFCs"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0970-6168","authenticated-orcid":false,"given":"Xinyue","family":"Li","sequence":"first","affiliation":[{"name":"Stomatological Hospital of Chongqing Medical University Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing 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