{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:19:52Z","timestamp":1788574792664,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of the present study was to evaluate the anti\u2010inflammatory effects of lipoxin A4 (LXA4) for the treatment of periodontitis in an in vitro model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human PDLCs were challenged with <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E. coli<\/jats:italic>) lipopolysaccharide (LPS) to evoke an inflammatory response. This was done either in monoculture or in coculture with THP\u20101, a monocytic cell line. Thereafter, cytokine expression was measured by ELISA, with or without LXA4. In addition, the effects of LXA4 were analyzed on the TLR\u2010MyD88\u2010NF\u2010\u03baB (TMN)\u2010mediated intracellular signal pathway using immunocytochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In response to LPS, the level of the pro\u2010inflammatory cytokine tumor necrosis factor alpha increased, whereas the anti\u2010inflammatory cytokine interleukin\u20104 decreased significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). These effects were consistently reversed when LPS\u2010challenged PDLCs were also treated with LXA4. The results in the coculture system were comparable to the monoculture. Immunohistochemistry and quantitative assessment confirmed the importance of the TMN signal pathway in these processes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results corroborate earlier findings that PDLCs play an important role in inflammation. Moreover, LXA4 might offer new approaches for the therapeutic treatment of periodontal disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13250","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T09:32:32Z","timestamp":1575883952000},"page":"429-438","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Lipoxin suppresses inflammation via the TLR4\/MyD88\/NF\u2010\u03baB pathway in periodontal ligament cells"],"prefix":"10.1111","volume":"26","author":[{"given":"Muhanad","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Dentistry \u2010 Biomaterials Radboud Institute for Molecular Life Sciences Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dentistry \u2010 Biomaterials Radboud Institute for Molecular Life Sciences Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Dentistry \u2010 Biomaterials Radboud Institute for Molecular Life Sciences Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5742-9826","authenticated-orcid":false,"given":"X. 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