{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:44:16Z","timestamp":1758635056611,"version":"3.40.5"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T00:00:00Z","timestamp":1580169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900966","81700933"],"award-info":[{"award-number":["81900966","81700933"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M663528"],"award-info":[{"award-number":["2019M663528"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DE026482"],"award-info":[{"award-number":["R01 DE026482"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Fibroblast growth factor 8 (FGF8) signaling is essential in regulating craniofacial osteogenesis. This study aims to explore the effect of altered FGF8 signaling in maxillomandibular development during embryogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p><jats:italic>Dmp1<\/jats:italic><jats:sup>Cre<\/jats:sup>;<jats:italic>R26R<\/jats:italic><jats:sup>mTmG<\/jats:sup> mice were generated to trace <jats:italic>Dmp1<\/jats:italic><jats:sup>+<\/jats:sup> cell lineage, and <jats:italic>Dmp1<\/jats:italic><jats:sup>Cre<\/jats:sup>;<jats:italic>R26R<\/jats:italic><jats:sup>Fgf8<\/jats:sup> mice were generated to explore the effects of augmented FGF8 signaling in <jats:italic>Dmp1<\/jats:italic><jats:sup>+<\/jats:sup> cells on osteogenesis with a focus on maxillomandibular development during embryogenesis, as assessed by whole mount skeletal staining, histology, and immunostaining. Additionally, cell proliferation rate and the expression of osteogenic genes were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Osteocytes of maxillomandibular bones were found <jats:italic>Dmp1<\/jats:italic>\u2010positive prenatally, and <jats:italic>Fgf8<\/jats:italic> over\u2010expression in <jats:italic>Dmp1<\/jats:italic><jats:sup>+<\/jats:sup> cells led to mandibular hypoplasia. While <jats:italic>Dmp1<\/jats:italic><jats:sup>Cre<\/jats:sup> allele functions in the osteocytes of the developing mandibular bone at as early as E13.5, and enhanced cell proliferation rate is observed in the bone forming region of the mandible in <jats:italic>Dmp1<\/jats:italic><jats:sup>Cre<\/jats:sup>;<jats:italic>R26R<\/jats:italic><jats:sup>Fgf8<\/jats:sup> mice at E14.5, histological examination showed that osteogenesis was initially impacted at E15.5, along with an inhibition of osteogenic differentiation markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Augmented FGF8 signaling in <jats:italic>Dmp1<\/jats:italic><jats:sup>+<\/jats:sup> cells lead to osteogenic deficiency in the mandibular bones, resulting in mandibular hypoplasia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13262","type":"journal-article","created":{"date-parts":[[2019,12,21]],"date-time":"2019-12-21T12:45:09Z","timestamp":1576932309000},"page":"590-596","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exogenous FGF8 signaling in osteocytes leads to mandibular hypoplasia in mice"],"prefix":"10.1111","volume":"26","author":[{"given":"Jue","family":"Xu","sequence":"first","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University Chengdu China"},{"name":"Department of Cell and Molecular Biology Tulane University New Orleans LA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Tulane University New Orleans LA USA"},{"name":"State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Disease, and Department of Preventive Dentistry West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Huang","sequence":"additional","affiliation":[{"name":"Southern Center for Biomedical Research and Fujian Key Laboratory of Developmental and Neuro Biology College of Life Sciences Fujian Normal University Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YiPing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Tulane University New Orleans LA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3384-9508","authenticated-orcid":false,"given":"Meiying","family":"Shao","sequence":"additional","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,28]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"2997","article-title":"Meckel\u2019s and condylar cartilages anomalies in achondroplasia result in defective development and growth of the mandible","volume":"25","author":"Biosse Duplan M.","year":"2016","journal-title":"Human Molecular Genetics"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.8.1671"},{"key":"e_1_2_8_4_1","first-page":"1","article-title":"Conditional deletion of Bmp2 in cranial neural crest cells recapitulates Pierre Robin sequence in mice","volume":"9","author":"Chen Y.","year":"2018","journal-title":"Cell and Tissue Research"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21534"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003231"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600404"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0298-136"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20335"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2005.01176.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.965702"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.11.025"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125328"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-237X.86467"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607956104"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjv052"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0479"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.23.3136"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1242\/dev.126.2.221","article-title":"Conserved regulation of mesenchymal gene expression by Fgf\u20108 in face and limb development","volume":"126","author":"Tucker A. 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