{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:03:23Z","timestamp":1792649003769,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,16]],"date-time":"2020-02-16T00:00:00Z","timestamp":1581811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570985"],"award-info":[{"award-number":["81570985"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>A diagnostic test was designed to evaluate the accuracy and applicability of fluorescence staining with fluorescein\u2010labelled chitinase versus routine 10% potassium hydroxide (KOH) smear for rapid diagnosis of oral candidiasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In total, 124 subjects with suspected oral candidiasis symptoms\/signs were sequentially enrolled in this study. The diagnostic efficacy indexes (sensitivity, specificity, Youden index, predictive value, likelihood ratio, diagnostic odds ratio, diagnostic accuracy and area under the curve [AUC]) were compared between 10% KOH smear and fluorescence staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The sensitivity (85.48% vs. 64.52%) and specificity (91.94% vs. 72.58%) of fluorescence staining were higher than those of KOH smear. The AUC of fluorescence staining (0.887) was remarkably higher than that of 10% KOH smear (0.685), demonstrating that the diagnostic efficacy of fluorescence staining is significantly higher than that of KOH smear (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0005). Furthermore, fluorescence staining showed higher sensitivity than that of KOH smear (84.75% vs. 62.71%) in diagnosis of erythematous type oral candidiasis, which is the most common type and the type most challenging to differentially diagnose.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The advantages of fluorescence staining with fluorescein\u2010labelled chitinase in rapid diagnosis of oral candidiasis and its ease of operation suggest its potential use in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13293","type":"journal-article","created":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T13:27:40Z","timestamp":1580736460000},"page":"941-947","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Fluorescence staining vs. routine KOH smear for rapid diagnosis of oral candidiasis\u2014A diagnostic test"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5196-5759","authenticated-orcid":false,"given":"Lijun","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Oral Medicine Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing China"},{"name":"Department of Oral Medicine The Affiliated Stomatology Hospital Zhejiang University School of Medicine Key Laboratory of Oral Biomedical Research of Zhejiang Province Zhejiang University School of Stomatology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiru","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0751-7374","authenticated-orcid":false,"given":"Zhimin","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Peking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(80)90147-8"},{"key":"e_1_2_9_3_1","first-page":"20","article-title":"Epidemiology of oropharyngeal Candidiasis in human immunodeficiency virus\/acquired immune deficiency syndrome patients and CD4+ counts","volume":"7","author":"Berberi A.","year":"2015","journal-title":"Journal of International Oral Health"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000325232"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-008-0240-6"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.326.7379.41"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.h5527"},{"key":"e_1_2_9_8_1","first-page":"487921","article-title":"Isolation and identification of Candida from the oral cavity","volume":"2011","author":"Byadarahally R. 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