{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:37:54Z","timestamp":1787067474207,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T00:00:00Z","timestamp":1594598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001702","name":"AO Foundation","doi-asserted-by":"publisher","award":["1406Z"],"award-info":[{"award-number":["1406Z"]}],"id":[{"id":"10.13039\/501100001702","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      In a previous rat model, MRONJ occurrence was 50%. Our aim was to investigate the potential of endothelial progenitor cells (EPCs) to improve fibroblasts function and prevent\n                      <jats:italic>MRONJ<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human gingival fibroblasts were cultured with EPC\u2010conditioned media (EPC\u2010CM); endothelial growth media (EGM\u20102) or DMEM followed by incubation with 10\u00a0\u00b5M zoledronic (ZOL) and dexamethasone (DEX). Cell proliferation and migration were assessed by XTT and scratch wound healing assays. In vivo, ten Lewis rats were treated weekly with ZOL and DEX for 11\u00a0weeks. After a week, EPCs or EGM\u20102 were injected to the gingiva around the molars. At 3\u00a0weeks, bilateral molars were extracted. After 8\u00a0weeks, wound healing was assessed, and serum VEGF and blood vessels were quantified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ZOL\/DEX significantly reduced fibroblasts proliferation and wound healing. Treatment with EPC\u2010CM before ZOL\/DEX improved cell proliferation, and scratch healing (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.007,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.023).\u00a0In vivo, local EPC injection before tooth extraction increased serum VEGF (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.01) and soft tissue vascularization (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.05). Normal healing was similar (80%) in EPCs and EGM\u20102 groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>EPC rescued fibroblasts from the cytotoxic effect of ZOL\/DEX and elevated serum VEGF and vessel density that might reduce MRONJ occurrence to 20% compared to 50% in a similar model.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.13412","type":"journal-article","created":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T06:34:29Z","timestamp":1589351669000},"page":"1523-1531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Endothelial progenitors increase vascularization and improve fibroblasts function that prevent medication\u2010related osteonecrosis of the jaw"],"prefix":"10.1111","volume":"26","author":[{"given":"Ofri","family":"Doppelt","sequence":"first","affiliation":[{"name":"Laboratory for Bone Repair Rambam Health Care Campus  Haifa Israel"},{"name":"The Ruth and Bruce Rappaport Faculty of Medicine Technion \u2010 Israel Institute of Technology  Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gal","family":"Cohen","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Repair Rambam Health Care Campus  Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tal","family":"Tamari","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Repair Rambam Health Care Campus  Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rina","family":"Elimelech","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Repair Rambam Health Care Campus  Haifa Israel"},{"name":"Department of Periodontology School of Graduate Dentistry Rambam Health Care Campus  Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadin","family":"Sabbah","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Repair Rambam Health Care Campus  Haifa Israel"},{"name":"The Ruth and Bruce Rappaport Faculty of Medicine Technion \u2010 Israel Institute of Technology  Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4940-6533","authenticated-orcid":false,"given":"Hadar","family":"Zigdon\u2010Giladi","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Repair Rambam Health Care Campus  Haifa Israel"},{"name":"The Ruth and Bruce Rappaport Faculty of Medicine Technion \u2010 Israel Institute of Technology  Haifa Israel"},{"name":"Department of Periodontology School of Graduate Dentistry Rambam Health Care Campus  Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5302.964"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2013.22.8.407"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2012.0398"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X557164"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201900226"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755-1536-1-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/009127002762491316"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070166"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/SCS.0b013e31824dbdd4"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-4096.2004.04831.x"},{"key":"e_1_2_8_12_1","first-page":"301","article-title":"Fibroblasts and myofibroblasts in wound healing","volume":"7","author":"Darby I. 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