{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:51:12Z","timestamp":1789480272424,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:00:00Z","timestamp":1594252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871413"],"award-info":[{"award-number":["31871413"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Benzo[a]pyrene (B[<jats:italic>a<\/jats:italic>]P) is a member of the polycyclic aromatic hydrocarbon (PAH) family. Although the potent carcinogenicity of high\u2010dose B[<jats:italic>a<\/jats:italic>]P has been extensively reported, the effects of long\u2010term exposure to B[<jats:italic>a<\/jats:italic>]P on the progression of tongue squamous cell carcinoma (TSCC) are poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the present study, TSCC cells were treated with 5 or 50\u00a0nM of B[<jats:italic>a<\/jats:italic>]P for three months. The proliferation and chemoresistance of B[<jats:italic>a<\/jats:italic>]P\u2010treated cells to 5\u2010fluorouracil or cisplatin were detected by CCK8. The motility of the B[<jats:italic>a<\/jats:italic>]P\u2010treated cells was evaluated with wound healing analysis, invasion assay, and three\u2010dimensional culture in decellularized mouse tongue matrix. Xenograft assay was used to investigate the aggressiveness of B[<jats:italic>a<\/jats:italic>]P\u2010treated cells. Immunofluorescence staining, terminal restriction fragment assay, and whole\u2010genome sequence were used to determine the mutation spectrums.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Long\u2010term 50\u00a0nM B[<jats:italic>a<\/jats:italic>]P\u2010treated cells exhibited increased aggressiveness and chemoresistance to 5\u2010fluorouracil or cisplatin. In addition, data from whole\u2010genome sequencing demonstrated that C:T to A:T transitions were the predominant nucleotide substitutions occurred in 50\u00a0nM B[<jats:italic>a<\/jats:italic>]P\u2010treated CAL27 cells. Furthermore, 102 non\u2010synonymous or stop\u2010gain mutations were enriched in the extracellular\u2010matrix\u2010receptor interactive pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>B[<jats:italic>a<\/jats:italic>]P exposure may contribute to genomic instability, and therefore, B[<jats:italic>a<\/jats:italic>]P may promote the progression of TSCC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13489","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T02:30:25Z","timestamp":1592533825000},"page":"1649-1658","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Benzo[<i>a<\/i>]pyrene promotes progression in tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"26","author":[{"given":"Linxuan","family":"Huang","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofen","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Stomatology Shenzhen People\u2019s Hospital 2nd Clinical Medical College of Jinan University Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yupeng","family":"Yao","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Yu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjian","family":"Chen","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puping","family":"Liang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3702-3401","authenticated-orcid":false,"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aag0299"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12477"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-018-0539-0"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3342"},{"key":"e_1_2_8_6_1","first-page":"113","article-title":"An examination of the time course from human dietary exposure to polycyclic aromatic hydrocarbons to urinary elimination of 1\u2010hydroxypyrene","volume":"49","author":"Buckley T. 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