{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:47:40Z","timestamp":1781246860429,"version":"3.54.1"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T00:00:00Z","timestamp":1596326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012807","name":"Midwestern University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare the effects of dietary fat and sex on murine oral squamous cell carcinoma pathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Male and female C57Bl\/6 mice (36\/sex) received a low\u2010fat (10\u00a0kcal%) or high\u2010fat (60\u00a0kcal%) diet. Water (control), vehicle, or 4\u2010nitroquinoline\u20101\u2010oxide in vehicle (50\u00a0\u03bcg\/ml) was provided for 17\u00a0weeks followed by six additional weeks of water. Oral lesion development was recorded weekly. Histopathologic changes in tongues were examined, and T cells (CD3+), macrophages (CD68+), and neutrophils (Ly6+) were quantified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All 4\u2010nitroquinoline\u20101\u2010oxide\u2010treated mice developed oral tumors. High\u2010fat diet exacerbated pathology, demonstrated by an increased final tumor burden (10.9\u00a0\u00b1\u00a04.5 vs. 7.9\u00a0\u00b1\u00a02.5, mm\/mouse, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05; high\u2010fat diet vs. low\u2010fat diet, respectively), and a greater histopathology score. When dietary groups were combined, 4\u2010nitroquinoline\u20101\u2010oxide\u2010treated males displayed higher histopathology scores than females (4.2\u00a0\u00b1\u00a00.3 vs. 3.6\u00a0\u00b1\u00a00.2, respectively, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Lymphoid cell infiltration was greater in the 4\u2010nitroquinoline\u20101\u2010oxide mouse tongues than controls: T cells (14.0 vs. 0.96\u00a0cells\/mm<jats:sup>2<\/jats:sup>), macrophages (3.6 vs. 1.8\u00a0cells\/mm<jats:sup>2<\/jats:sup>), and neutrophils (12.0 vs. 0.38\u00a0cells\/mm<jats:sup>2<\/jats:sup>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>High\u2010fat diet and male sex increased the pathology of 4\u2010nitroquinoline\u20101\u2010oxide\u2010induced oral cancer. Elevated lymphoid cell infiltration contributed to disease pathology.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13542","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T23:41:17Z","timestamp":1594251677000},"page":"215-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Dietary fat and male sex increase histopathological changes in a mouse model of oral cancer"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4966-7178","authenticated-orcid":false,"given":"Jacalyn M.","family":"Green","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics College of Graduate Studies Midwestern University Downers Grove IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mae J.","family":"Ciancio","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Program College of Graduate Studies 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