{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T03:53:17Z","timestamp":1788234797180,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T00:00:00Z","timestamp":1616371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The aim of this study was to test the efficacy of autoantibodies to desmoglein 1 and desmoglein 3 detected by ELISA and indirect immunofluorescence in the diagnosis of oral pemphigus and to correlate the antibody titres with the severity of the disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>We report a retrospective cohort study of 22 patients with oral pemphigus and 64 controls from a single tertiary centre. Data about histopathological examination, direct immunofluorescence, indirect immunofluorescence and ELISA were analysed. Global validation of ELISA and IIF both alone and combined was established by calculating sensitivity, specificity, accuracy and both positive predictive value and negative predictive value. The relationship between Oral Disease Severity Score values and ELISA titres was analysed using Pearson's coefficient.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The best diagnostic performance was observed for anti\u2010desmoglein 3 ELISA. The sensitivity was 75% and specificity 100% and positive predictive value and negative predictive value were 92.5% and accuracy 93.9%. The level of agreement with histopathology\u00a0+\u00a0direct immunofluorescence was substantial (\n                      <jats:italic>k<\/jats:italic>\n                      \u00a0=\u00a0.758). Anti\u2010desmoglein 3 titres showed a significant correlation with Oral Disease Severity Score (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Serological tests are commonly employed during clinical practice as adjunctive tools. Anti\u2010desmoglein 3 ELISA should be considered as a first\u2010instance diagnostic test for oral pemphigus early detection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.13849","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T13:41:45Z","timestamp":1615815705000},"page":"1149-1156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["ELISA detection of anti\u2010desmoglein 1 and anti\u2010desmoglein 3 and indirect immunofluorescence in oral pemphigus: A retrospective study"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9030-3739","authenticated-orcid":false,"given":"Massimo","family":"Petruzzi","sequence":"first","affiliation":[{"name":"Interdisciplinary Department of Medicine University of Bari \u201cAldo Moro\u201d  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2677-0869","authenticated-orcid":false,"given":"Alberta","family":"Lucchese","sequence":"additional","affiliation":[{"name":"Multidisciplinary Department of Medical Surgical and Odontostomatological Specialties University of Campania \u201cLuigi Vanvitelli,\u201d Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Contaldo","sequence":"additional","affiliation":[{"name":"Multidisciplinary Department of Medical Surgical and Odontostomatological Specialties University of Campania \u201cLuigi Vanvitelli,\u201d Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilina","family":"Tampoia","sequence":"additional","affiliation":[{"name":"Section of Clinical Pathology Policlinico Hospital  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Antonia","family":"Frassanito","sequence":"additional","affiliation":[{"name":"Section of Clinical Pathology Policlinico Hospital  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3550-1812","authenticated-orcid":false,"given":"Dorina","family":"Lauritano","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Center of Neuroscience Milan University of \u201cMilan Bicocca\u201d  Monza Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6467-2039","authenticated-orcid":false,"given":"Fedora","family":"della Vella","sequence":"additional","affiliation":[{"name":"Interdisciplinary Department of Medicine University of Bari \u201cAldo Moro\u201d  Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2009.9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(99)70183-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01190"},{"key":"e_1_2_9_5_1","first-page":"112","article-title":"Oral manifestations of pemphigus vulgaris: Clinical presentation, differential diagnosis and management","volume":"1","author":"Bascones\u2010Martinez A.","year":"2010","journal-title":"Journal of Clinical and Experimental Dermatology Research"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2019.02.005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2008.00686.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-016-0343-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.13052"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.4317\/jced.56289"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.5826\/dpc.1003a50"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00779"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2014.130428"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2009.03408.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04132.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0868"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00266"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1961"},{"key":"e_1_2_9_20_1","unstructured":"Ministero della Salute Italiano. 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