{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:06:11Z","timestamp":1789725971932,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008731","name":"Nakatomi Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004330","name":"Smoking Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004330","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the role of hypoxia\u2010inducible factor 1\u03b1 (HIF\u20101\u03b1) signaling, the expression profile of M1 and M2 macrophages, and the role of the sphingosine 1\u2010phosphate (S1P)\/S1P receptor system in palatal wound healing of heterozygous HIF\u20101\u03b1\u2010deficient (HIF\u20101\u03b1 HET) mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>HIF\u20101\u03b1 HET and wild\u2010type (WT) littermates underwent palatal tissue excision at the mid\u2010hard palate. Histological analysis, immunostaining, real\u2010time PCR, Western blotting (WB), and cellular migration assays were performed to analyze wound closure and macrophage infiltration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      DMOG pretreatment showed an acceleration of palatal wound closure in WT mice. In contrast, the delayed palatal wound closure was observed in HIF\u20101\u03b1 HET mice with diminished production of Col1a1, MCP\u20101, and MIP\u20101\u03b1, compared with WT mice. Decreased infiltration of M1 macrophage (F4\/80\n                      <jats:sup>+<\/jats:sup>\n                      TNF\u2010\u03b1\n                      <jats:sup>+<\/jats:sup>\n                      , F4\/80\n                      <jats:sup>+<\/jats:sup>\n                      iNOS\n                      <jats:sup>+<\/jats:sup>\n                      ) and M2 macrophage (F4\/80\n                      <jats:sup>+<\/jats:sup>\n                      Arginase\u20101\n                      <jats:sup>+<\/jats:sup>\n                      , F4\/80\n                      <jats:sup>+<\/jats:sup>\n                      CD163\n                      <jats:sup>+<\/jats:sup>\n                      ) was observed. The numbers of F4\/80\n                      <jats:sup>+<\/jats:sup>\n                      S1P\n                      <jats:sub>1<\/jats:sub>\n                      <jats:sup>+<\/jats:sup>\n                      macrophages of HIF\u20101\u03b1 HET wounded tissues were significantly lower compared with WT tissues. S1P treatment of bone marrow macrophages (BMMs) significantly upregulated expression of S1P\n                      <jats:sub>1<\/jats:sub>\n                      in WT mice compared with HIF\u20101\u03b1 HET. Phosphorylation of MAPK rapidly decreased in BMMs of HIF\u20101\u03b1 HET mice than in BMMs of WT mice by S1P stimulation. Moreover, S1P enhanced HIF\u20101\u03b1 expression via S1P\n                      <jats:sub>1<\/jats:sub>\n                      receptors to affect macrophage migration.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      HIF\u20101\u03b1 deficiency aggravates M1 and M2 macrophage infiltration and controls macrophage motility via S1P\/S1P\n                      <jats:sub>1<\/jats:sub>\n                      signaling. These results suggest that HIF\u20101\u03b1 signaling may contribute to the regulation of palatal wound healing.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.13856","type":"journal-article","created":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T23:50:03Z","timestamp":1616543403000},"page":"1157-1169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["HIF\u20101\u03b1 controls palatal wound healing by regulating macrophage motility via S1P\/S1P\n                    <sub>1<\/sub>\n                    signaling axis"],"prefix":"10.1111","volume":"28","author":[{"given":"Islamy Rahma","family":"Hutami","sequence":"first","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics Institute of Biomedical Sciences Tokushima University Graduate School  Tokushima Japan"},{"name":"Department of Orthodontics Faculty of Dentistry Sultan Agung Islamic University  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