{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T10:12:25Z","timestamp":1780654345249,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The role of Human papillomavirus (HPV) in the oral squamous cell carcinoma (OSCC) has not been completely elucidated. The purpose of the present study was to investigate the prevalence and localization of HPV\u201016 virus in OSCC and to correlate HPV\u201016 positivity and p16INK4A expression with the clinical and pathological features of OSCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The archives of Oral Pathology at the University of Florida, College of Dentistry were accessed for demographic, clinical, histopathological data, and slides of 114 OSCC patients. HPV\u201016 positivity of OSCC was evaluated by p16INK4A immunohistochemistry (IHC) and HPV\u201016 E6\/E7mRNA by in situ hybridization (ISH).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Out of 114 consecutive pathological slides of OSCC, 16\u00a0samples (14%) showed positivity for p16INK4A by IHC and 14\u00a0samples (12%) were positive for HPV\u201016 E6\/E7mRNA ISH and the Positivity showed a significant correlation with the patients' age, alcohol consumption, and the degree of OSSC differentiation. The hard palate showed the highest positivity of p16INK4A IHC and HPV\u201016\u00a0mRNA ISH (38%, 36% respectively).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>HPV\u201016 is a significant factor in oral carcinogenesis. We recommend using p16INK4A as a surrogate marker for HPV detection in OSCC, which can be complemented by RNA ISH for the identification of HPV subtypes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.13920","type":"journal-article","created":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T12:49:53Z","timestamp":1621687793000},"page":"436-444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Localization and characterization of human papillomavirus\u201016 in oral squamous cell carcinoma"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4143-7084","authenticated-orcid":false,"given":"Wafaa","family":"Saleh","sequence":"first","affiliation":[{"name":"Oral Diagnostic Sciences Department College of Dentistry University of Florida  Gainesville FL USA"},{"name":"Oral Medicine and Periodontology Department Faculty of Dentistry Mansoura University  Mansoura FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seunghee","family":"Cha","sequence":"additional","affiliation":[{"name":"Oral Diagnostic Sciences Department College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz","family":"Banasser","sequence":"additional","affiliation":[{"name":"Oral Diagnostic Sciences Department College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah G.","family":"Fitzpatrick","sequence":"additional","affiliation":[{"name":"Oral Diagnostic Sciences Department College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indraneel","family":"Bhattacharyya","sequence":"additional","affiliation":[{"name":"Oral Diagnostic Sciences Department College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilan M.","family":"Youssef","sequence":"additional","affiliation":[{"name":"Oral Medicine and Periodontology Department Faculty of Dentistry Mansoura University  Mansoura FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed M.","family":"Anees","sequence":"additional","affiliation":[{"name":"Oral Medicine and Periodontology Department Faculty of Dentistry Mansoura University  Mansoura FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Islam\u00a0A.","family":"Elzahaby","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Mansoura Oncology Center Mansoura University  Mansoura FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0741-8149","authenticated-orcid":false,"given":"Joseph","family":"Katz","sequence":"additional","affiliation":[{"name":"Oral Diagnostic Sciences Department College of Dentistry University of Florida  Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2020.104746"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/2057178X16647973"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12649"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318265fb2b"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.55"},{"issue":"4","key":"e_1_2_9_7_1","first-page":"889","article-title":"Role of human papilloma virus in oral tongue squamous cell carcinoma","volume":"12","author":"Elango K. 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