{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:53:47Z","timestamp":1772160827588,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004376","name":"Terry Fox Research Institute","doi-asserted-by":"publisher","award":["2009\u201024"],"award-info":[{"award-number":["2009\u201024"]}],"id":[{"id":"10.13039\/501100004376","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>High\u2010risk human papillomavirus (HR\u2010HPV) can cause oropharyngeal squamous cell carcinoma (OpSCC). The revised 8th edition of the AJCC Staging Manual now stages OpSCC by incorporating p16 immunohistochemistry (IHC), the surrogate marker for HPV status. This study assessed the prognostic values of p16 and HPV markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We identified 244 OpSCC patients diagnosed between 2000 and 2008 from the British Columbia Cancer Registry with enough tissue to conduct experiments. Formalin\u2010fixed, paraffin\u2010embedded tissue sections were stained for p16 IHC, RNA in situ hybridization (ISH) HPV 16 and 18, and DNA ISH HR\u2010HPV. Electronic charts were reviewed to collect clinical and outcome data. Combined positive RNA and\/or DNA ISH was used to denote HPV status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Human papillomavirus was positive among 77.9% of samples. Using HPV as the benchmark, p16 IHC had high sensitivity (90.5%), but low specificity (68.5%). Distinct subgroups of patients were identified by sequential separation of p16 then HPV status. Among both p16\u2010positive and p16\u2010negative groups, HPV\u2010positive patients were younger, more males, and had better clinical outcomes, especially 5\u2010year overall survival. We further evaluated the technical costs associated with HPV testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Human papillomavirus is more prognostic than p16 for OpSCC. Clinical laboratories can adopt HPV RNA ISH for routine analysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.13938","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T14:44:34Z","timestamp":1623768274000},"page":"483-490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prognostic value and cost benefit of HPV testing for oropharyngeal cancer patients"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7583-2651","authenticated-orcid":false,"given":"Xian Jun David","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Oral Biological and Medical Sciences Faculty of Dentistry University of British Columbia  Vancouver BC Canada"},{"name":"Department of Integrative Oncology British Columbia Cancer Research Centre  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly Yi Ping","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral Biological and Medical Sciences Faculty of Dentistry University of British Columbia  Vancouver BC Canada"},{"name":"Department of Integrative Oncology British Columbia Cancer Research Centre  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eitan","family":"Prisman","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology\u2010Head and Neck Surgery Department of Surgery Vancouver General Hospital  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonn","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology British Columbia Cancer Agency, Vancouver Center  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqi Sarah","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Integrative Oncology British Columbia Cancer Research Centre  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Poh","sequence":"additional","affiliation":[{"name":"Department of Oral Biological and Medical Sciences Faculty of Dentistry University of British Columbia  Vancouver BC Canada"},{"name":"Department of Integrative Oncology British Columbia Cancer Research Centre  Vancouver BC Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.164806.113"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20936"},{"key":"e_1_2_9_4_1","volume-title":"AJCC cancer staging manual","author":"Amin M. 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