{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T21:58:00Z","timestamp":1785794280566,"version":"3.56.0"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Host immunity is crucial during periodontal inflammations. B cells are considered to have a function of immunoregulation, and TLRs are considered to be crucial in this process. The present study illustrates the potential roles and rules of CD25+ B cells during periodontitis, especially its effect on regulating host IL\u201035 level and Th1, Th17, and Treg differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>The proportion of local and systemic CD25+ B cell subpopulations from periodontitis models were identified by flow cytometry. To illustrate further mechanism, B cells were cultured with a different type of TLR activators. Expression of IL\u201010, IL\u201035, and TGF\u2010\u03b2 was detected by ELISA and real\u2010time PCR. We also set adoptive transfer models by using CD25+ B cells. Alveolar bone erosion, proportion of Th1, Th17, and Tregs, and levels of IFN\u2010\u03b3, TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u201017 were identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Periodontitis induces more CD25+ B cell subpopulations and promotes their IL\u201010, IL\u201035, and TGF\u2010\u03b2production. TLR activators enhanced Breg proliferation and function. LPS+CpG obviously induced more CD25+ B cell differentiation and production of IL\u201010, IL\u201035, and TGF\u2010\u03b2. Adoptive transfer of CD25+ B cells reduces alveolar bone destruction and local Tregs, proportion, especially the local level of IFN\u2010\u03b3 and IL\u201017. In addition, adoptive transfer of CD25+ B cells remedies the pathological change in the proportion of IL\u20101\u03b2 and Th1\/Th17 in local lesions. We did not find any significant difference in peripheral blood, regardless of group and detected items.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Results of the present study clarify that CD25+ B cells enlarged and produced more IL\u201010, IL\u201035 and TGF\u2010\u03b2 during periodontitis, activation of TLR4 and TLR9 played crucial roles in this process. Also, CD25+ B cells alleviated periodontal inflammation and alveolar bone resorption. Our findings further expanded the potential of B cells during periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.13939","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T17:43:36Z","timestamp":1623779016000},"page":"2248-2257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["CD25+ B cells produced IL\u201035 and alleviated local inflammation during experimental periodontitis"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8232-1080","authenticated-orcid":false,"given":"Yakun","family":"Han","sequence":"first","affiliation":[{"name":"Department of Periodontology The Fourth Hospital of Harbin Medical University  Harbin China"},{"name":"Department of Stomatology Affiliated Hospital of Jilin Medical University  Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Jilin Medical University  Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Jilin Medical University  Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangjia","family":"Bi","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Fourth Hospital of Harbin Medical University  Harbin 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