{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:14:59Z","timestamp":1790190899472,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY13H140001"],"award-info":[{"award-number":["LY13H140001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Many activities overload temporomandibular joint (TMJ) and cause mandibular condylar cartilage (MCC) degradation by inducing the expression of hypoxia\u2010inducible factor\u20102\u03b1 (HIF\u20102\u03b1). Although NF\u2010\u03baB signaling pathway has been reported to induce HIF\u20102\u03b1 expression, the underlying mechanisms need to be verified. The aim was to investigate the effects of NF\u2010\u03baB\/HIF\u20102\u03b1 on MCC degradation induced by mechanical stress and the regulatory mechanism of NF\u2010\u03baB in the HIF\u20102\u03b1 pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Chondrocytes were subjected to cyclic compressive forces in a hypoxic environment. Western blotting was used to test the effects of stress on the expression of NF\u2010\u03baB and HIF\u20102\u03b1.\n                      <jats:italic>HIF<\/jats:italic>\n                      \u2010\n                      <jats:italic>2\u03b1<\/jats:italic>\n                      siRNA and shRNA were constructed and transfected into MCC cells\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      to inhibit HIF\u20102\u03b1 expression. To test the regulatory effect of the NF\u2010\u03baB pathway on HIF\u20102\u03b1, siRNA\n                      <jats:italic>p65<\/jats:italic>\n                      was transfected into MCC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results showed that mechanical stress could cause cartilage degradation and significantly increased the expression of NF\u2010\u03baB, HIF\u20102\u03b1, and downstream degradation factors (MMP13 and ADAMTs\u20104). Blockade of HIF\u20102\u03b1 decreased cartilage degradation and related degradation factors. Suppression of p65\u00a0significantly decreased the expression of HIF\u20102\u03b1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results indicated that the upstream NF\u2010\u03baB pathway exerted a regulatory effect on HIF\u20102\u03b1 in the degradation of MCC induced by stress.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.13986","type":"journal-article","created":{"date-parts":[[2021,8,3]],"date-time":"2021-08-03T03:28:06Z","timestamp":1627961286000},"page":"2239-2247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Role of HIF\u20102\u03b1\/NF\u2010\u03baB pathway in mechanical stress\u2010induced temporomandibular joint osteoarthritis"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2039-6420","authenticated-orcid":false,"given":"Wen","family":"Li","sequence":"first","affiliation":[{"name":"Stomatology Hospital School of Stomatology Zhejiang University School of Medicine Clinical Research Center for Oral Diseases of Zhejiang Province Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8450-4091","authenticated-orcid":false,"given":"Na","family":"Wu","sequence":"additional","affiliation":[{"name":"Stomatology Hospital School of Stomatology Zhejiang University School of Medicine Clinical Research Center for Oral Diseases of Zhejiang Province Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junming","family":"Wang","sequence":"additional","affiliation":[{"name":"Experimental Teaching Center of Basic Medicine Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingnan","family":"Wang","sequence":"additional","affiliation":[{"name":"Stomatology Hospital School of Stomatology Zhejiang University School of Medicine Clinical Research Center for Oral Diseases of Zhejiang Province Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"Wu","sequence":"additional","affiliation":[{"name":"Stomatology Hospital School of Stomatology Zhejiang University School of Medicine Clinical Research Center for Oral Diseases of Zhejiang Province Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0851-8539","authenticated-orcid":false,"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"Experimental Teaching Center of Basic Medicine Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.09.086"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201009491\u20105"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2015.2434"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8070734"},{"key":"e_1_2_10_6_1","first-page":"1","article-title":"Association between self\u2010reported sleep bruxism and temporomandibular disorder in undergraduate students from Brazil, Cranio","volume":"26","author":"Emidgio","year":"2018","journal-title":"Journal of Cranio\u2010Mandibular Practice"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102842.1986.tb01301.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100039\u20101697938"},{"key":"e_1_2_10_9_1","first-page":"164","article-title":"Prevalence of malocclusion, its association with occlusal interferences and temporomandibular disorders among the Saudi sub\u2010population","volume":"13","author":"Haralur S. 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