{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:35:53Z","timestamp":1789472153747,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2008\/57471\u20107"],"award-info":[{"award-number":["2008\/57471\u20107"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["303064\/2018\u20108"],"award-info":[{"award-number":["303064\/2018\u20108"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the potential effect of fatty acid synthase (FASN) inhibitor orlistat to enhance the effectiveness of chemotherapy drugs widely used to treat oral squamous cell carcinomas (OSCC), such as 5\u2010fluorouracil, cisplatin, and paclitaxel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The OSCC SCC\u20109 LN\u20101\u00a0metastatic cell line, which expresses high levels of FASN, was used for drug combination experiments. Cell viability was analyzed by crystal violet staining and automatic cell counting. Apoptosis and cell cycle were analyzed by flow cytometry with Annexin\u2010V\/7\u2010AAD and propidium iodide staining, respectively. Cyclin B1, Cdc25C, Cdk1, FASN, and ERBB2\u00a0levels were assessed by Western blotting. Finally, cell scratch and transwell assays were performed to assess cell migration and invasion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Inhibition of FASN with orlistat sensitized SCC\u20109 LN\u20101 cells to the cytotoxic effects of paclitaxel and cisplatin, but not 5\u2010fluorouracil, which was accompanied by a significant reduction in cyclin B1. The suppression of proliferation, migration, and invasion of SCC\u20109 LN\u20101 cells induced by orlistat plus cisplatin or paclitaxel was not superior to the effects of chemotherapy drugs alone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggest that orlistat enhances the chemosensitivity of SCC\u20109 LN\u20101 cells to cisplatin and paclitaxel by downregulating cyclin B1.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.14017","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T17:22:20Z","timestamp":1631467340000},"page":"649-660","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["FASN inhibition sensitizes metastatic OSCC cells to cisplatin and paclitaxel by downregulating cyclin B1"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-4052","authenticated-orcid":false,"given":"Luciana Yamamoto de","family":"Almeida","sequence":"first","affiliation":[{"name":"Department of Oral Diagnosis Piracicaba Dental School State University of Campinas (UNICAMP)  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda dos Santos","family":"Moreira","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis Piracicaba Dental School State University of Campinas (UNICAMP)  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8441-4512","authenticated-orcid":false,"given":"Guilherme Augusto Silva dos","family":"Santos","sequence":"additional","affiliation":[{"name":"School of Medicine University of Ribeir\u00e3o Preto (UNAERP)  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence Juana Maria","family":"Cuadra Zelaya","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis Piracicaba Dental School State University of Campinas (UNICAMP)  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3420-803X","authenticated-orcid":false,"given":"C\u00e9sar\u00a0Alexander","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Hematology Division, Department of Medical Images, Hematology, and Clinical Oncology University of S\u00e3o Paulo at Ribeir\u00e3o Preto Medical School  Ribeir\u00e3o\u00a0Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3648-337X","authenticated-orcid":false,"given":"Michelle","family":"Agostini","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis and Pathology, School of Dentistry Federal University of Rio de Janeiro (UFRJ)  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via Sammartino","family":"Mariano","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis Piracicaba Dental School State University of Campinas (UNICAMP)  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2700-3669","authenticated-orcid":false,"given":"D\u00e9bora\u00a0Campanella","family":"Bastos","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Piracicaba Dental School State University of Campinas (UNICAMP)  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulisses Ribaldo Nicolau","family":"Daher","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, A. 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