{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:48:14Z","timestamp":1790858894826,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T00:00:00Z","timestamp":1636675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800982"],"award-info":[{"award-number":["81800982"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972072"],"award-info":[{"award-number":["81972072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to clarify the regulatory role of Th17\u2010Treg balance in periodontitis and further reveal Treg plasticity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>An experimental periodontitis model was established by ligation and injection of Pg\u2010LPS. Inflammatory factors were measured by ELISA and RT\u2010PCR. Alveolar bone absorption was evaluated by micro\u2010CT and histomorphology. Quantities of Treg and Th17 cell and their related gene expression were examined. Furthermore, after magnetic bead\u2010sorting spleen Treg cells, Treg\/Th17 characteristic genes were explored. Immunofluorescence double staining of Foxp3 and IL\u201017 was conducted to further reveal Treg plasticity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Inflammatory cytokines in serum and gingival tissue increased significantly in periodontitis, which revealed obvious crestal bone loss. Further analysis showed that the number of Th17 cells and expression of related genes increased more significantly than Treg cells, demonstrating Treg\u2010Th17 imbalance. Flow cytometry showed that the proportions of Treg cells in the blood and spleen were lower in periodontitis group. Furthermore, Foxp3 was downregulated, and Rorc\/ IL\u201017A were increased in Treg cells of periodontitis group. Immunofluorescence double staining showed significantly increased number of IL\u201017+Foxp3+ cells in periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results provided evidence that Treg cells showed characteristics of Th17 cells in mice with periodontitis, although its mechanisms require further study.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14072","type":"journal-article","created":{"date-parts":[[2021,11,6]],"date-time":"2021-11-06T01:50:38Z","timestamp":1636163438000},"page":"1149-1162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Regulatory T cells showed characteristics of T helper\u201017(Th17) cells in mice periodontitis model"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9500-6637","authenticated-orcid":false,"given":"Yuying","family":"Kou","sequence":"first","affiliation":[{"name":"Department of Bone Metabolism School and Hospital of Stomatology Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujun","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Bone Metabolism School and Hospital of Stomatology Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration  Jinan Shandong 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