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Biofilm analysis included quantification of extracellular matrix components (water\u2010soluble and insoluble polysaccharides, proteins and extracellular DNA), and biomass (total and insoluble), as well as the enumeration of colony\u2010forming units. The data were analyzed using three\u2010way analysis of variance with Bonferroni's post hoc test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The DNase treatment combined with aPDT showed a reduction of 1.92, 1.65, and 1.29\u00a0log<jats:sub>10<\/jats:sub> of cell viability compared with untreated controls for ATCC 96901, R14, and R70\u00a0strains, respectively. It also reduced extracellular matrix contents of water\u2010soluble polysaccharides (36.3%) and extracellular DNA (72.3%), as well as insoluble biomass content (43.3%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The three strains showed similar behavior when treated with DNase, and the extracellular matrix components were affected, improving the effectiveness of antimicrobial photodynamic therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.14149","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T14:02:46Z","timestamp":1644328966000},"page":"1855-1867","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["DNase enhances photodynamic therapy against fluconazole\u2010resistant <i>Candida albicans<\/i> biofilms"],"prefix":"10.1111","volume":"29","author":[{"given":"C\u00e9sar Augusto","family":"Abreu\u2010Pereira","sequence":"first","affiliation":[{"name":"Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara Univ Estadual Paulista \u2010 UNESP  Araraquara Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7916-1557","authenticated-orcid":false,"given":"Marlise In\u00eaz","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara Univ Estadual Paulista \u2010 UNESP  Araraquara Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carm\u00e9lia Isabel Vitorino","family":"Lobo","sequence":"additional","affiliation":[{"name":"Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara Univ Estadual Paulista \u2010 UNESP  Araraquara Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luiza","family":"Gorayb 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