{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T16:49:11Z","timestamp":1788194951375,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T00:00:00Z","timestamp":1647561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900992"],"award-info":[{"award-number":["81900992"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900992"],"award-info":[{"award-number":["81900992"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Osteoblasts suppress osteoclastogenesis during the reversal phase of bone remodelling and the mechanism needs to be further investigated. Here, we investigated the role of histone demethylase Jumonji domain\u2010containing 3 (Jmjd3) in osteoblasts on regulating osteoclastogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Jmjd3 expression was silenced in osteoblasts. Osteoblasts and osteoclasts were co\u2010cultured in direct or indirect contact ways, and osteoclastogenesis was determined by tartrate\u2010resistant acid phosphatase (TRAP) staining and Western blotting. Additionally, Ephrin receptor B4 (EphB4) and receptor activator of nuclear factor\u2010kappa \u0392 ligand (RANKL) expression were quantified in osteoblasts via real\u2010time PCR, Western blotting, and enzyme\u2010linked immunosorbent assay. Subsequently, EphB4 was overexpressed in osteoblasts and RANKL expression and osteoclastogenesis was quantified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Osteoclastogenesis and marker protein expression levels was promoted when osteoclasts were co\u2010cultured with Jmjd3\u2010silenced osteoblasts. Silencing of Jmjd3 expression in osteoblasts decreased EphB4 expression, owing to suppression of demethylation of H3K27me3 on the promoter region of EphB4. Whereas RANKL expression was upregulated in Jmjd3\u2010silenced osteoblasts. Overexpression of EphB4 in osteoblasts inhibited osteoclastogenesis and RANKL expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Jmjd3 in osteoblasts is a crucial regulator of osteoblast\u2010to\u2010osteoclast communication through EphB4\u2013EphrinB2, RANKL\u2013RANK and EphB4\u2013RANKL signalling axes, suggesting the pivotal role of Jmjd3 in bone remodelling process in bone destruction disease such as chronic apical periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14160","type":"journal-article","created":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T21:14:12Z","timestamp":1645218852000},"page":"1613-1621","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Osteoblast Jmjd3 regulates osteoclastogenesis via EphB4 and RANKL signalling"],"prefix":"10.1111","volume":"29","author":[{"given":"Rui","family":"Wang","sequence":"first","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Oral Disease Department of Endodontics School and Hospital of Stomatology China Medical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huikun","family":"Luo","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Oral Disease Department of Endodontics School and Hospital of Stomatology China Medical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4615-2897","authenticated-orcid":false,"given":"Di","family":"Yang","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Oral Disease Department of Endodontics School and Hospital of Stomatology China Medical University  Shenyang 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