{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:59:35Z","timestamp":1792159175829,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T00:00:00Z","timestamp":1646179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["2018R1A5A2024418"],"award-info":[{"award-number":["2018R1A5A2024418"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to identify formation of tubular dentin induced by transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) and bone morphogenic protein (BMP) signaling pathway in dental epithelial cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We collected conditioned medium (CM) of rTGF\u2010\u03b21\/rBMP\u20102\u2010treated HAT\u20107 and treated to MDPC\u201023 cells. The expression levels of odontoblast differentiation markers, KLF4, DMP1, and DSP were evaluated by real\u2010time PCR and Western blot analysis. To evaluate whether CM of rTGF\u2010\u03b21\/rBMP\u20102 induces tubular dentin formation, we made a beagle dog tooth defect model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Here, we show that Cpne7 is regulated by Smad4\u2010dependent TGF\u2010\u03b21\/BMP2 signaling pathway in dental epithelial cells. CM of rTGF\u2010\u03b21\/rBMP\u20102 treated HAT\u20107 or rCPNE7 raises the expression levels of KLF4, DMP1, and DSP in MDPC\u201023 cells. When rTGF\u2010\u03b21 or rBMP\u20102 is directly treated to MDPC\u201023 cells, however, expression levels of Cpne7\u2010regulated genes remain unchanged. In a beagle dog defect model, application of rTGF\u2010\u03b21\/BMP2\u2010treated CM resulted in tubular tertiary dentin mixed with osteodentin at cavity\u2010prepared sites, while rTGF\u2010\u03b21 group exhibited homogenous osteodentin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Taken together, Smad4\u2010dependent TGF\u2010\u03b21\/BMP2 signaling regulates Cpne7 in dental epithelial cells, and CPNE7 protein secreted from pre\u2010ameloblasts mediates odontoblast differentiation via epithelial\u2013mesenchymal interaction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14170","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T04:37:58Z","timestamp":1645677478000},"page":"1644-1656","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Tubular dentin formation by TGF\u2010\u03b2\/BMP signaling in dental epithelial cells"],"prefix":"10.1111","volume":"29","author":[{"given":"Yoon Seon","family":"Lee","sequence":"first","affiliation":[{"name":"Laboratory for the Study of Regenerative Dental Medicine Department of Oral Histology\u2010Developmental Biology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3766-7274","authenticated-orcid":false,"given":"Yeoung\u2010Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory for the Study of Regenerative Dental Medicine Department of Oral Histology\u2010Developmental Biology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"},{"name":"Regenerative Dental Medicine R and D Center Hysensbio Co., Ltd  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You\u2010Mi","family":"Seo","sequence":"additional","affiliation":[{"name":"Laboratory for the Study of Regenerative Dental Medicine Department of Oral Histology\u2010Developmental Biology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye\u2010Kyung","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory for the Study of Regenerative Dental Medicine Department of Oral Histology\u2010Developmental Biology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3162-7557","authenticated-orcid":false,"given":"Joo\u2010Cheol","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory for the Study of Regenerative Dental Medicine Department of Oral Histology\u2010Developmental Biology School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,2]]},"reference":[{"issue":"3","key":"e_1_2_9_2_1","first-page":"405","article-title":"Comparative analysis of tgf beta s, bmps, igf1, msxs, fibronectin, osteonectin and bone sialoprotein gene expression during normal and in vitro\u2010induced odontoblast differentiation","volume":"38","author":"Begue\u2010Kirn C.","year":"1994","journal-title":"The International Journal of Developmental Biology"},{"issue":"4","key":"e_1_2_9_3_1","first-page":"491","article-title":"Effects of dentin proteins, transforming growth factor beta 1 (tgf beta 1) and bone morphogenetic protein 2 (bmp2) on the differentiation of odontoblast in vitro","volume":"36","author":"Begue\u2010Kirn C.","year":"2004","journal-title":"International Journal of Developmental Biology"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.4.1371"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709492200"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516639919"},{"issue":"1","key":"e_1_2_9_7_1","first-page":"41","article-title":"Mineral trioxide aggregate produces superior outcomes in vital primary molar pulpotomy","volume":"32","author":"Doyle T. 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