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Clinical diagnosis was categorized as either non\u2010syndromic cleft lip with or without cleft palate (NSCL\/P), or non\u2010syndromic cleft palate only (NSCPO). Tissues excised from the trimmed wound edge were reserved as experimental samples; adjacent normal control was used as a positive control, and tissue from healthy individuals was used as a blank control. Target lncRNAs in the collected tissues were identified using microarrays and quantitative reverse transcription PCR (RT\u2010qPCR). Immunohistochemical (IHC) staining and RT\u2010qPCR were used to verify the target mRNAs. Pathway, gene ontology (GO) enrichment, and TargetScan predictions were employed to construct competing endogenous RNA networks (ceRNA networks) and explore their potential functions. RNA\u2010Seq revealed 24 upregulated and 43 downregulated lncRNAs; MALAT1 and NEAT1 were screened and validated using RT\u2010qPCR. Common NSOFC risk factors were positively correlated with MALAT1 and NEAT1 expression. Bioinformatics predicted four ceRNA networks; GO enrichment focused on their potential functions. RT\u2010qPCR and IHC data were consistent with respect to expression levels of proteins and the mRNAs that encode them. As MALAT1 and NEAT1 are associated with the severity of NSOFC, they represent potential therapeutic targets and prognostic biomarkers.<\/jats:p>","DOI":"10.1111\/odi.14177","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T15:59:36Z","timestamp":1646668776000},"page":"1668-1679","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Long non\u2010coding RNAs MALAT1 and NEAT1 in non\u2010syndromic orofacial clefts"],"prefix":"10.1111","volume":"29","author":[{"given":"Errui","family":"Wang","sequence":"first","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumeng","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Gao","sequence":"additional","affiliation":[{"name":"Applied Oral Sciences &amp; Community Dental Care Faculty of Dentistry The University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"},{"name":"Gansu Province Key Lab of Maxillofacial Reconstruction and Intelligent Manufacturing  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waruna Lakmal","family":"Dissanayaka","sequence":"additional","affiliation":[{"name":"Applied Oral Sciences &amp; Community Dental Care Faculty of Dentistry The University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yayuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaozhen","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junkai","family":"Zhai","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengkun","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4468-5091","authenticated-orcid":false,"given":"Baoping","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"},{"name":"Gansu Province Key Lab of Maxillofacial Reconstruction and Intelligent Manufacturing  Lanzhou China"},{"name":"Institute of Biomechanics and Medical Engineering Lanzhou University Key Laboratory of Mechanics on Disaster and Environment in Western China Ministry of Education Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruimin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Gansu Province Hospital  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5661-3979","authenticated-orcid":false,"given":"Kailiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Stomatology Lanzhou University  Lanzhou China"},{"name":"Gansu Province Key Lab of Maxillofacial Reconstruction and Intelligent Manufacturing  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,16]]},"reference":[{"issue":"3","key":"e_1_2_9_2_1","first-page":"348","article-title":"Association of genetic variation of the transforming growth factor\u2010alpha gene with cleft lip and palate","volume":"45","author":"Ardinger H. 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