{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T20:47:56Z","timestamp":1786394876615,"version":"build-2736575974"},"reference-count":74,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T00:00:00Z","timestamp":1650067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Oral tongue squamous cell carcinoma (OTSCC) represents the most common malignancy of the oral cavity. Tumor budding (TB) is a reliable prognostic factor in OTSCC; however, a standardized scoring system is not still validated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>The study aims to evaluate the prognostic role of TB in 211 OTSCC patients treated between 1997 and 2018.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials &amp; Methods<\/jats:title>\n                    <jats:p>\n                      TB was evaluated on hematoxylin and eosin\u2010stained sections in the hotspot area of the infiltrative front (\u00d7200\u2010magnification). It was scored using a two\u2010tier system, a three\u2010tier system, and according to BD\u2010model and revised\u2010Grading system. Univariate and multivariate Cox regression analyses of disease\u2010specific survival (DSS) and disease\u2010free survival (DFS) were performed. A\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05 was considered as statistically significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The two\u2010tier and three\u2010tier systems resulted an independent prognostic factor of DSS. High\u2010risk patients had a 2.21 and 3.08 times increased probability of poor DSS compared with low\u2010risk group. It is significantly increased even for intermediate\u2010risk group. No significant differences emerged classifying patients according to BD\u2010model and revised\u2010Grading system.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These data confirm the prognostic value of TB in predicting DSS in OTSCC. Classifying patients into two groups using the 5\u2010bud cutoff significantly discriminates their outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Since the established role of DOI and the poor prognostic value of grading, TB could be considered an independent prognostic marker.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14193","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T15:10:46Z","timestamp":1647961846000},"page":"1947-1958","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Prognostic significance of tumor budding thresholds in oral tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7304-3752","authenticated-orcid":false,"given":"Marco","family":"Mascitti","sequence":"first","affiliation":[{"name":"Department of Clinical Specialistic and Dental Sciences Marche Polytechnic University  Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4580-8594","authenticated-orcid":false,"given":"Lucrezia","family":"Togni","sequence":"additional","affiliation":[{"name":"Department of Clinical Specialistic and Dental Sciences Marche Polytechnic University  Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5080-5921","authenticated-orcid":false,"given":"Vito Carlo Alberto","family":"Caponio","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Foggia University  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khrystyna","family":"Zhurakivska","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Foggia University  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4633-4893","authenticated-orcid":false,"given":"Lorenzo","family":"Lo Muzio","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Foggia University  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0632-8632","authenticated-orcid":false,"given":"Corrado","family":"Rubini","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health Marche Polytechnic University  Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4218-3354","authenticated-orcid":false,"given":"Andrea","family":"Santarelli","sequence":"additional","affiliation":[{"name":"Department of Clinical Specialistic and Dental Sciences Marche Polytechnic University  Ancona Italy"},{"name":"National Institute of Health and Science of Ageing IRCCS INRCA  Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-4414","authenticated-orcid":false,"given":"Giuseppe","family":"Troiano","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Foggia University  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,16]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Cancer staging manual","author":"AJCC","year":"2017"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.23380"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2014.10.004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-017-2212-1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2017.425"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12471"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13793"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-10-51"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1066896914565022"},{"issue":"11","key":"e_1_2_10_11_1","first-page":"6111","article-title":"Tumor budding, EMT and cancer stem cells in T1\u20132\/N0 oral squamous cell carcinomas","volume":"35","author":"Attramadal C. 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