{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:53:46Z","timestamp":1772160826807,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,6,5]],"date-time":"2022-06-05T00:00:00Z","timestamp":1654387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Tight junctions (TJs) are involved in the regulation of salivary secretion via paracellular pathway. Botulinum toxin type A (BTXA) is widely used for the treatment of hypersecretion diseases such as sialorrhea. This study aimed to investigate the role of TJs in BTXA\u2010inhibited secretion of the submandibular gland (SMG).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>BTXA was injected into the SMGs of rats, and the same amount of saline was injected as a control. Western blot, real\u2010time PCR, and immunofluorescence staining were used to detect the expression and distribution of TJ proteins. Paracellular permeability was evaluated using the transepithelial electrical resistance (TER) measurements and fluorescent tracer detection in BTXA\u2010stimulated SMG\u2010C6 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>BTXA injection into the SMGs of rats led to increased expression of claudin (Cldn) \u22121 and Cldn3. Immunofluorescence staining showed no significant changes in the distribution of TJ proteins. In vitro, BTXA increased the TER values and significantly reduced the permeability of fluorescent tracer, suggesting that BTXA decreased the paracellular permeability. The expression levels of Cldn1, Cldn3, and Cldn4 were upregulated after BTXA treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The expression of TJ proteins changed in both animal models and SMG\u2010C6 cells after BTXA treatment, which may contribute to the inhibition of salivary secretion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14223","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T13:28:11Z","timestamp":1650979691000},"page":"2086-2095","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Alteration of tight junctions during botulinum toxin type A\u2010inhibited salivary secretion"],"prefix":"10.1111","volume":"29","author":[{"given":"Qian\u2010Ying","family":"Mao","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shang","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Peking University School of Basic Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5935-1911","authenticated-orcid":false,"given":"Ruo\u2010Lan","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Peking University School of Basic Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7657-8843","authenticated-orcid":false,"given":"Zhi\u2010Gang","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/21688370.2016.1162348"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00144.2008"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.07.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2012.12"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00182.2003"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27362"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200110122"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24761"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00019.2012"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2015.08.014"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.13203"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins12110691"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40120-019-00155-6"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.507"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2008.951780"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2015.07.002"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2010.07.014"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-019-09854-8"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/joor.12664"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2015.08.014"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12116"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-997-0137-8"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12887"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599812465059"},{"key":"e_1_2_10_26_1","first-page":"38","article-title":"The mechanism of inhibitory effect on parotid gland secretion with local injection of botulinum toxin type A in the rat","volume":"44","author":"Wen W. 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