{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:15:07Z","timestamp":1790792107937,"version":"4.1.0"},"reference-count":74,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T00:00:00Z","timestamp":1652400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901029"],"award-info":[{"award-number":["81901029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700938"],"award-info":[{"award-number":["81700938"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001030"],"award-info":[{"award-number":["82001030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017596","name":"Natural Science Basic Research Program of Shaanxi Province","doi-asserted-by":"publisher","award":["2020JQ\u2010560"],"award-info":[{"award-number":["2020JQ\u2010560"]}],"id":[{"id":"10.13039\/501100017596","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016112","name":"Xi'an Science and Technology Association","doi-asserted-by":"publisher","award":["20YXYJ0010(1)"],"award-info":[{"award-number":["20YXYJ0010(1)"]}],"id":[{"id":"10.13039\/501100016112","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["xzy012020110"],"award-info":[{"award-number":["xzy012020110"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Mammalian palatogenesis is a highly regulated morphogenetic process to form the intact roof of the oral cavity. Long noncoding RNAs (lncRNAs) and mRNAs participate in numerous biological and pathological processes, but their roles in palatal development and causing orofacial clefts (OFC) remain to be clarified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Palatal tissues were separated from ICR mouse embryos at four stages (E10.5, E13.5, E15, and E17). Then, RNA sequencing (RNA\u2010seq) was used. Various analyses were performed to explore the results. Finally, hub genes were validated via qPCR and in situ hybridization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Starting from E10.5, the expression of cell adhesion genes escalated in the following stages. Cilium assembly and ossification genes were both upregulated at E15 compared with E13.5. Besides, the expression of cilium assembly genes was also increased at E17 compared with E15. Expression patterns of three lncRNAs (\n                      <jats:italic>H19<\/jats:italic>\n                      ,\n                      <jats:italic>Malat1<\/jats:italic>\n                      , and\n                      <jats:italic>Miat<\/jats:italic>\n                      ) and four mRNAs (\n                      <jats:italic>Cdh1<\/jats:italic>\n                      ,\n                      <jats:italic>Irf6<\/jats:italic>\n                      ,\n                      <jats:italic>Grhl3<\/jats:italic>\n                      ,\n                      <jats:italic>Efnb1<\/jats:italic>\n                      ) detected in RNA\u2010seq were validated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study provides a time\u2010series expression landscape of mRNAs and lncRNAs during palatogenesis, which highlights the importance of processes such as cell adhesion and ossification. Our results will facilitate a deeper understanding of the complexity of gene expression and regulation during palatogenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14237","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T03:16:19Z","timestamp":1651634179000},"page":"2163-2176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Time\u2010series expression profiles of mRNAs and lncRNAs during mammalian palatogenesis"],"prefix":"10.1111","volume":"29","author":[{"given":"Wenbin","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology  Beijing China"},{"name":"National Center of Stomatology National Clinical Research Center for Oral Diseases National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and\u2010 3 \u2010Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Zhong","sequence":"additional","affiliation":[{"name":"National Center of Stomatology National Clinical Research Center for Oral Diseases National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and\u2010 3 \u2010Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials Peking University School and Hospital of Stomatology  Beijing China"},{"name":"The Affiliated Stomatology Hospital Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"He","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology School of Basic Medical Sciences Xi\u2019an Jiaotong University Health Science Center  Xi\u2019an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhu","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of Stomatology Xi\u2019an Jiaotong University  Xi'an China"},{"name":"Department of Orthodontics College of Stomatology Xi\u2019an Jiaotong University  Xi\u2019an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuxiang","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology  Beijing China"},{"name":"National Center of Stomatology National Clinical Research Center for Oral Diseases National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and\u2010 3 \u2010Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology School of Basic Medical Sciences Xi\u2019an Jiaotong University Health Science Center  Xi\u2019an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9661-0166","authenticated-orcid":false,"given":"Huaxiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of Stomatology Xi\u2019an Jiaotong University  Xi'an China"},{"name":"Department of Orthodontics College of Stomatology Xi\u2019an Jiaotong University  Xi\u2019an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Shanghai Ninth People\u2019s Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"National Clinical Research Center for Oral Disease  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1899-8051","authenticated-orcid":false,"given":"Yunfei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology  Beijing China"},{"name":"National Center of Stomatology National Clinical Research Center for Oral Diseases National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and\u2010 3 \u2010Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials Peking University School and Hospital of Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/org.27375"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-2634"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3598"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.067082"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1113"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2018.04.009"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2017.08.017"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30039"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2933"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.071"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.080473"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-5-1-r1"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti1022"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-19-0203"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327119899996"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327116651121"},{"key":"e_1_2_9_21_1","first-page":"676","article-title":"LncRNA H19 and target gene\u2010mediated cleft palate induced by TCDD","volume":"30","author":"Gao L. Y.","year":"2017","journal-title":"Biomedical and Environmental Sciences : BES"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1613"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2013.09.012"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006610"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.08.021"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-322780"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29132"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4979"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-5203-3_1"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.157750"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10697"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0201-4"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-559"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt087"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2020.05.028"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517703580"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0125013"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034519851786"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23457"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2021.01.017"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517726284"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28369-7"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.592271"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20684"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08520-7"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.12.016"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-113"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20735"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2020.103438"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2017.03.015"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5248604"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71946"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2019.07.013"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.9.4520"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00165"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418\u2010011\u20100876\u20101"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418\u2010012\u20100915\u20106"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2019.02.22"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2019.11.93"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/mgg3.522"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-00315-9"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002010309"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3031"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809579106"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds497"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz444"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003785"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2484"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-010-1731-7"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.36670"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1597\/15-178"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.23859"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2019.12.010"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-013-9986-y"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.14237","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/odi.14237","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.14237","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T17:24:17Z","timestamp":1692465857000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.14237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,13]]},"references-count":74,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1111\/odi.14237"],"URL":"https:\/\/doi.org\/10.1111\/odi.14237","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/ODI.14237\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v1\/review4","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v2\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14237\/v2\/response1","asserted-by":"object"}]},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,13]]},"assertion":[{"value":"2021-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}