{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:01:45Z","timestamp":1787162505041,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T00:00:00Z","timestamp":1660608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M663530"],"award-info":[{"award-number":["2019M663530"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900981"],"award-info":[{"award-number":["81900981"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Chondrogenic differentiation of human dental pulp stem cells (hDPSCs) is highly promising for cartilage repair. The specific mechanism, however, still needs to be explicated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>In this study, we isolated hDPSCs and transfected cells with lentiviruses containing an over\u2010expression, knock\u2010down, or negative control of miR\u201020a\u20105p. Three\u2010D pellet cultures of hDPSCs were used for the chondrogenic induction. Following the pellet culture period, chondrogenesis was assessed by histological and immunohistochemical analysis and expression of chondrogenic\u2010related genes. Dual\u2010luciferase report assay was performed to determine potential targeted genes of miR\u201020a\u20105p, and the phosphorylation levels of P65 and I\u03baB\u03b1 were explored. Animal experiments were performed to determine the effect of miR\u201020a\u20105p on cartilage regeneration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>miR\u201020a\u20105p was showed to repress the expression of SMAD6 to inhibit the chondrogenic differentiation of hDPSCs. Accordingly, the knock\u2010down of miR\u201020a\u20105p promoted cartilage regeneration in the osteochondral defects of rats. Mechanically, it is indicated that NF\u2010\u03baB signaling is the potential down\u2010stream network of miR\u201020a\u20105p\/Smad6 crosstalk during chondrogenic differentiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>miR\u201020a\u20105p could target SMAD6 to activate NF\u2010\u03baB signaling pathway, and thus inhibit chondrogenesis of hDPSCs, which provided promising therapeutic target for cartilage defects clinically.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14331","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T15:46:21Z","timestamp":1659455181000},"page":"3433-3446","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>miR<\/scp>\n                    \u201020a\u20105p regulated\n                    <scp>SMAD6<\/scp>\n                    to inhibit chondrogenesis of\n                    <scp>hDPSCs<\/scp>"],"prefix":"10.1111","volume":"29","author":[{"given":"Xuefeng","family":"Pan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2762-2438","authenticated-orcid":false,"given":"Xinqi","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Pei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2955-1706","authenticated-orcid":false,"given":"Zhihe","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3959-1608","authenticated-orcid":false,"given":"Xiao","family":"Cen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Department of Temporomandibular Joint, West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology, Zhejiang Engineering Research Center for Biomedical Materials, Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9450240"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(04)00045\u20105"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2007.02.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis\u20102011\u2010200377"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.035"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010021\u201002501\u20108"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1383"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.117.029703"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb434"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.81"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2019.1593094"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnh001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010018\u20100892\u20103"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2010.05.030"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.03.025"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)80303\u20107"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.356.6343.1134\u2010r"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.02.050"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.08.027"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/09636897211052959"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/39355"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.06.023"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119682"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3562"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1379643"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1469"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v039a08"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000506304"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.01.001"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1350705"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.07.011"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.030338"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02\u201007\u20100441"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/39369"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388\u2010019\u20101083\u20100"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12094\u2010018\u20101910\u20108"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/1941738109350438"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.150399"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13164096"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2008.07.003"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2124"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.219"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100100107"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9020423"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2017056117"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.09.044"},{"issue":"9","key":"e_1_2_9_48_1","first-page":"646","article-title":"Burden of major musculoskeletal conditions","volume":"81","author":"Woolf A. D.","year":"2003","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.11.025"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201045502\u20107"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20180921"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010020\u201001791\u20108"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.179"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.11.028"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.12.012"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00894"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.14331","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/odi.14331","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.14331","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T08:42:59Z","timestamp":1721896979000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.14331"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,16]]},"references-count":55,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1111\/odi.14331"],"URL":"https:\/\/doi.org\/10.1111\/odi.14331","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/ODI.14331\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v2\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14331\/v2\/review1","asserted-by":"object"}]},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,16]]},"assertion":[{"value":"2022-04-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}