{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:45:30Z","timestamp":1788576330716,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371145"],"award-info":[{"award-number":["81371145"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771084"],"award-info":[{"award-number":["81771084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of the study was to determine the anti\u2010osteoclastogenic potential of ginsenoside Rb3 for the treatment of periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The anti\u2010osteoclastogenic effect was determined using RANKL\u2010induced RAW264.7 cells and murine bone marrow\u2010derived macrophages followed by TRAP and phalloidin staining. Expression of osteoclastogenesis\u2010related genes and proteins were examined by qPCR and WB. Activation of signaling pathways was detected by WB and IHC techniques. Experimental periodontitis rat model was built up by gingival injections of\n                      <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                      LPS. After 21\u2009days of Rb3 treatment, rats were sacrificed for micro\u2010CT, IHC, H&amp;E, and TRAP staining analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Rb3 dramatically inhibits RANKL\u2010induced osteoclastogenesis.\n                      <jats:italic>Nfatc1, Mmp9, Ctsk, Acp5<\/jats:italic>\n                      mRNA, and MMP9, CTSK proteins were dose\u2010dependently downregulated by Rb3 pretreatment. WB results revealed that Rb3 suppressed activations of p38 MAPK, ERK, and p65 NF\u2010\u03baB, and the inhibition of ERK was most pronounced. Consistently, IHC analysis revealed that p\u2010ERK was highly expressed in alveolar bone surface, blood vessels, odontoblasts, and gingival epithelia, which were notably suppressed by Rb3 treatment. H&amp;E staining and micro\u2010CT analyses showed that Rb3 significantly attenuated gingivitis and alveolar bone resorption in rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Rb3 inhibits RANKL\u2010induced osteoclastogenesis and attenuates\n                      <jats:italic>P.\u00a0gingivalis<\/jats:italic>\n                      LPS\u2010induced gingivitis and alveolar bone resorption in rats via ERK\/NF\u2010\u03baB signaling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14352","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T09:30:02Z","timestamp":1660728602000},"page":"3460-3471","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Ginsenoside Rb3 inhibits osteoclastogenesis via\n                    <scp>ERK<\/scp>\n                    \/\n                    <scp>NF\u2010\u03baB<\/scp>\n                    signaling pathway in vitro and in vivo"],"prefix":"10.1111","volume":"29","author":[{"given":"Minmin","family":"Sun","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Engineering Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"School of Stomatology Weifang Medical University  Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoting","family":"Ji","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Engineering Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan 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