{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:09:33Z","timestamp":1791227373862,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,11,13]],"date-time":"2022-11-13T00:00:00Z","timestamp":1668297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671020"],"award-info":[{"award-number":["81671020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970970"],"award-info":[{"award-number":["81970970"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001089"],"award-info":[{"award-number":["82001089"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Apical periodontitis is the most frequently occurring pathological lesion. Fat mass and obesity\u2010associated protein (Fto) is the first identified RNA N6\u2010methyladenosine demethylase. However, whether Fto regulates apical periodontitis remains unclear. This study aimed to explore the mechanisms of Fto in the tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1)\u2010induced inflammatory response.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      We established an apical periodontitis model. An immortalized cementoblast cell line (OCCM\u201030) cells were exposed to TNF\u2010\u03b1.\n                      <jats:italic>Fto<\/jats:italic>\n                      ,\n                      <jats:italic>Il6<\/jats:italic>\n                      ,\n                      <jats:italic>Mcp1<\/jats:italic>\n                      , and\n                      <jats:italic>Mmp9<\/jats:italic>\n                      expressions were assessed by qRT\u2010PCR. We knocked down\n                      <jats:italic>Fto<\/jats:italic>\n                      using lentiviruses and detected TNF\u2010\u03b1\u2010induced inflammation\u2010related gene expressions and mRNA stability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mice with apical periodontitis showed downregulation of Fto expression. OCCM\u201030 cells exposed to TNF\u2010\u03b1 showed an upregulation of inflammation\u2010related genes with a decrease in\n                      <jats:italic>Fto<\/jats:italic>\n                      . Furthermore, knockdown of\n                      <jats:italic>Fto<\/jats:italic>\n                      promoted the expressions of\n                      <jats:italic>Il6<\/jats:italic>\n                      ,\n                      <jats:italic>Mcp1<\/jats:italic>\n                      , and\n                      <jats:italic>Mmp9<\/jats:italic>\n                      in TNF\u2010\u03b1\u2010treated OCCM\u201030 cells as compared with negative control cells, whereas it did not affect the mRNA stability. Interestingly,\n                      <jats:italic>Fto<\/jats:italic>\n                      knockdown activated the p65, p38, and ERK1\/2 pathways, and it slightly activated the JNK signaling pathway after TNF\u2010\u03b1 administration in OCCM\u201030 cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      A TNF\u2010\u03b1\u2010induced decrease in the expression of\n                      <jats:italic>Fto<\/jats:italic>\n                      might play a critical role in the inflammatory response in cementoblasts, and knockdown of\n                      <jats:italic>Fto<\/jats:italic>\n                      might upregulate the inflammatory response.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14396","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T11:48:42Z","timestamp":1665661722000},"page":"2806-2815","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<scp>\n                      m\n                      <sup>6<\/sup>\n                      A\n                    <\/scp>\n                    demethylase Fto regulates the\n                    <scp>TNF<\/scp>\n                    \u2010\u03b1\u2010induced inflammatory response in cementoblasts"],"prefix":"10.1111","volume":"29","author":[{"given":"Biao","family":"Li","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyuan","family":"Du","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Orthodontics, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Sun","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4261-9034","authenticated-orcid":false,"given":"Zhengguo","family":"Cao","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9234-0015","authenticated-orcid":false,"given":"Hong","family":"He","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Orthodontics, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12062"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2012.02105.x"},{"key":"e_1_2_9_4_1","first-page":"390","volume-title":"Are Cementoblasts a subpopulation of osteoblasts or a unique phenotype?","author":"Bosshardt D. 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