{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:22:58Z","timestamp":1787242978362,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960204"],"award-info":[{"award-number":["81960204"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860198"],"award-info":[{"award-number":["81860198"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,4]]},"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n To explore the protective effect of human urine\u2010derived stem cell exosomes (hUSC\u2010Exos) on radiation\u2010induced salivary gland (SG) injuries in Sprague Dawley rats.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Fresh adult urine was collected, and primary hUSCs were isolated and identified. The hUSCs were hypoxia\u2010pretreated with 1% oxygen for 24\u2009h and then transferred to a normoxic culture environment for 24\u2009h. The hUSC\u2010Exos were collected and identified for exosomes. A radiation\u2010induced injury model was established in the rats, and exosomes were introduced by local injection in the SG and tail vein. The submandibular gland was excised for morphological observation 1\u2009week later. Immunohistochemical detection of the glandular tissue was conducted by \u03b1\u2010smooth muscle actin (a\u2010SMA), stem cell growth factor receptor (c\u2010Kit) staining, and periodic acid\u2013Schiff staining. Qualitative polymerase chain reaction and western blot analysis were adopted to detect the gene and protein expression of\n Wnt3a<\/jats:italic>\n ,\n GSK3\u03b2<\/jats:italic>\n , and\n Axin<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n In both the normoxic and hypoxic hUSC\u2010Exo groups, microvesicular structures with bilayer membranes of approximately 80\u2009nm in diameter were detected, and the expressions of\n CD9<\/jats:italic>\n and\n CD63<\/jats:italic>\n were detected by nanoflow cytometry. Compared with the control group, in the radiation\u2010induced injury model group, the expression of a\u2010SMA was significantly higher, the expression of c\u2010Kit was significantly lower, and the expressions of\n Wnt3a<\/jats:italic>\n ,\n GSK3\u03b2<\/jats:italic>\n , and\n Axin<\/jats:italic>\n were significantly upregulated; the differences were statistically significant (\n p<\/jats:italic>\n \u2009<\u20090.05). Compared with the model group, in the normoxic and hypoxic hUSC\u2010Exo groups, the expression of a\u2010SMA was significantly decreased, the expression of c\u2010Kit was significantly increased, and the expressions of\n Wnt3a<\/jats:italic>\n ,\n GSK3\u03b2<\/jats:italic>\n , and\n Axin<\/jats:italic>\n were significantly upregulated; the differences were statistically significant (\n p<\/jats:italic>\n \u2009<\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Hypoxia\u2010pretreated hUSC\u2010Exos could repair radiation\u2010induced SG injuries by activating the\n Wnt3a\/GSK3\u03b2<\/jats:italic>\n pathway to suppress the expressions of a\u2010SMA and c\u2010Kit.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/odi.14476","type":"journal-article","created":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T08:41:33Z","timestamp":1671698493000},"page":"1234-1241","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Repair mechanism of\n radiation\u2010induced<\/scp>\n salivary gland injury by\n hypoxia\u2010pretreated<\/scp>\n human urine\u2010derived stem cell exosomes"],"prefix":"10.1111","volume":"30","author":[{"given":"Xiang\u2010Yang","family":"Xiao","sequence":"first","affiliation":[{"name":"Medical College of Soochow University Soochow China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni\u2010Ni","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dentofacial Surgery Affiliated Stomatological Hospital of Zunyi Medical University Zunyi 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