{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:08:51Z","timestamp":1777100931260,"version":"3.51.4"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:00:00Z","timestamp":1684454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012403","name":"Central University Basic Scientific Research Business Expenses Special Funds","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012403","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to investigate the effect of mammalian\u2010enabled (Mena) on tongue squamous cell carcinoma (TSCC) metastasis and its mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Immunochemistry was performed to investigate the Mena and tumor\u2010related markers expression, and its clinicopathological characteristics in 46 TSCC specimens. TSCC cell SCC9 and Cal27 untransfected or stable transfected with Mena overexpression and small interfering RNA were used to determine the role of Mena in cell proliferation, cell migration, invasion and metastasis, and EMT\u2010related markers in vitro, and the effect of Mena on TSCC growth and metastasis through tumor\u2010bearing and tumor metastasis immunodeficient mice models in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunochemistry showed that the expression of Mena was significantly correlated with lymphatic metastasis and TNM stage, E\u2010cadherin, Vimentin, and MMP2. Mena did not affect cell proliferation and colony formation in vitro, and tumor growth in vivo. However, it promoted cell migration and invasion in vitro, and TSCC metastasis in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Mena expression is associated with lymphatic metastasis and tumor stage and promotes TSCC invasion and metastasis by inducing the EMT process. Thus, Mena may be a biomarker for prognosis and targeted therapy in TSCC patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.14616","type":"journal-article","created":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T08:58:44Z","timestamp":1684486724000},"page":"2084-2096","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mena as a key enhancer factor of <scp>EMT<\/scp> to promote metastasis of human tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"30","author":[{"given":"Hao","family":"Cui","sequence":"first","affiliation":[{"name":"Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Department of Oral and Maxillofacial Surgery, College of Stomatology Xi'an Jiaotong University  Xi'an Shaanxi 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