{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T02:28:59Z","timestamp":1783736939827,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T00:00:00Z","timestamp":1686182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Periodontitis is an inflammatory disease caused by bacteria present in the dental biofilm. However, the presence of two oral protozoans,\n                      <jats:italic>Entamoeba gingivalis<\/jats:italic>\n                      and\n                      <jats:italic>Trichomonas tenax<\/jats:italic>\n                      , in patients with the periodontal disease remains largely unknown in Taiwan. Therefore, we investigated the prevalence of oral microbial infections between the sites with mild gingivitis and chronic periodontitis in patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>We collected 60 dental biofilm samples from sites with mild gingivitis (probing depth\u2009&lt;5\u2009mm) and chronic periodontitis (probing depth\u2009\u22655\u2009mm) from 30 patients at the National Cheng Kung University Hospital. The samples were analyzed via polymerase chain reaction and gel electrophoresis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among oral protozoans,\n                      <jats:italic>E. gingivalis<\/jats:italic>\n                      and\n                      <jats:italic>T. tenax<\/jats:italic>\n                      were detected in 44 (74.07%) and 14 (23.33%) of all samples, respectively. Among oral bacteria,\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      ,\n                      <jats:italic>Treponema denticola<\/jats:italic>\n                      , and\n                      <jats:italic>Tannerella forsythia<\/jats:italic>\n                      were detected in 50 (83.33%), 47 (78.33%), and 48 (80.0%) samples, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study, which is the first to analyze\n                      <jats:italic>E. gingivalis<\/jats:italic>\n                      and\n                      <jats:italic>T. tenax<\/jats:italic>\n                      presence among patients with periodontitis in Taiwan, revealed an association between periodontitis and oral microbes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14638","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T06:14:02Z","timestamp":1686204842000},"page":"1483-1487","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparative prevalence of oral bacteria and protozoa in patients with periodontitis in Taiwan"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5435-4134","authenticated-orcid":false,"given":"Yu Chun","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5621-488X","authenticated-orcid":false,"given":"Jian Ming","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Medicine National Tsing Hua University  Hsinchu Taiwan"},{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University  Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying\u2010Ying","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Stomatology National Cheng Kung University Hospital  Tainan Taiwan"},{"name":"School of Dentistry &amp; Institute of Oral Medicine, College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7174-8540","authenticated-orcid":false,"given":"Kuo","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Stomatology National Cheng Kung University Hospital  Tainan Taiwan"},{"name":"School of Dentistry &amp; Institute of Oral Medicine, College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7223-5931","authenticated-orcid":false,"given":"Wei Chen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology College of Medicine, National Cheng Kung University  Tainan Taiwan"},{"name":"Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University  Tainan Taiwan"},{"name":"Department of Parasitology, College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1304-2918","authenticated-orcid":false,"given":"Chun Chan","family":"Ting","sequence":"additional","affiliation":[{"name":"Department of Stomatology National Cheng Kung University Hospital  Tainan Taiwan"},{"name":"School of Dentistry &amp; Institute of Oral Medicine, College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000177"},{"key":"e_1_2_6_3_1","first-page":"906","article-title":"Incidence of Entamoeba Gingivalis and trichomonas Tenax in periodontitis and gingivitis patients who attended to private Clincs in Babylon Province","volume":"20","author":"Al\u2010Dulaimi F. 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A.","year":"2020","journal-title":"Medico Legal Update"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.248"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1996.tb00180.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/3130607"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2019.1693222"},{"key":"e_1_2_6_8_1","first-page":"101","article-title":"The prevalence of Entamoeba gingivalis and trichomonas tenax in periodontal disease","volume":"22","author":"El Hayawan I.","year":"1992","journal-title":"Journal of the Egyptian Society of Parasitology"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-018-5990-8"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-018-5798-6"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2020.08.001"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4118-9"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1965.36.3.177"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2312-9"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-019-0644-3"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.14638","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T08:42:40Z","timestamp":1721896960000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.14638"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,8]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1111\/odi.14638"],"URL":"https:\/\/doi.org\/10.1111\/odi.14638","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/ODI.14638\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14638\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14638\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14638\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14638\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14638\/v2\/review2","asserted-by":"object"}]},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,8]]},"assertion":[{"value":"2023-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}