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Adeno\u2010associated virus was used to knock down\n                      <jats:italic>Rip3<\/jats:italic>\n                      and the effect of\n                      <jats:italic>Rip3<\/jats:italic>\n                      knockdown on periodontal inflammation was examined by Micro\u2010CT, qRT\u2010PCR and histological staining. In vitro,\n                      <jats:italic>P.\u2009gingivalis<\/jats:italic>\n                      \u2010LPS was used to infect fibroblast cell line L929 and siRNA was used to knock down\n                      <jats:italic>Rip3<\/jats:italic>\n                      . Necroptosis pathway signalling and inflammation in cells were detected by cell viability and death assay, Western Blot, qRT\u2010PCR and immunofluorescence analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Phosphorylation of RIP3 and mixed lineage kinase domain\u2010like protein (MLKL) was increased in the periodontal ligament of mice infected with\n                      <jats:italic>P.\u2009gingivalis<\/jats:italic>\n                      . RIP3 knockdown reduced osteoclastogenesis and inflammatory cytokines in the periodontal area, and alleviated alveolar bone loss in vivo. In vitro,\n                      <jats:italic>P.\u2009gingivalis<\/jats:italic>\n                      \u2010LPS\u2010induced RIP3\u2010mediated necroptosis in L929 cells, and knockdown of RIP3 by siRNA decreased the expression of inflammatory cytokines.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>RIP3\u2010mediated necroptosis is activated in periodontitis and blocking necroptosis alleviates disease progression, indicating that RIP3 may be a potential target for periodontitis treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14693","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:40:32Z","timestamp":1690764032000},"page":"2485-2496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Activation of 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