{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T10:16:17Z","timestamp":1784024177290,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>During the development of temporomandibular joint osteoarthritis, endochondral ossification is compromised which leads to condylar degeneration; miR\u2010335\u20105p in endochondral ossification in osteoarthritic condylar cartilage tissue remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Up\u2010regulated microRNA and its target gene were searched for endochondral ossification in osteoarthritis articular cartilage. The effect of increased or decreased miR\u2010335\u20105p on endochondral ossification was evaluated by transfecting miR\u2010335\u20105p mimics or miR\u2010335\u20105p inhibitor in vitro in chondrocytes C28\/I2. Finally, we injected the temporomandibular joint of rats intra\u2010articularly with agomiR\u2010335 in a unilateral anterior crossbite rat model to determine the in vivo regulation of miR\u2010335.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After the onset of temporomandibular joint osteoarthritis, miR\u2010335\u20105p levels were gradually up\u2010regulated, whereas endochondral ossification\u2010related genes were down\u2010regulated in condylar cartilage specimens. Our results showed that miR\u2010335 inhibited endochondral ossification after administration of a miR\u2010335 antagonist into the temporomandibular joint articular cavity of a unilateral anterior crossbite rat model. AgomiR\u2010335, a miR\u2010335 agonist, inhibited matrix mineralization in fibrocartilage stem cells in vitro and then miR\u2010335\u20105p negatively regulated chondrocyte activity by directly targeting SP1 via promoting transforming growth factor\u2010\u03b2\/Smad signalling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>miR\u2010335\u20105p can significantly inhibit endochondral ossification; therefore, its inhibition may be beneficial for the treatment of temporomandibular joint osteoarthritis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.14736","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T07:59:31Z","timestamp":1695196771000},"page":"3176-3187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>miR<\/scp>\u2010335\u20105p inhibits endochondral ossification by directly targeting <scp>SP1<\/scp> in <scp>TMJ OA<\/scp>"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7785-7016","authenticated-orcid":false,"given":"Simo","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Oral Surgery, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2839-4649","authenticated-orcid":false,"given":"Pei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.11543"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345211037248"},{"key":"e_1_2_9_4_1","first-page":"1581","article-title":"SP1\/TGF\u2010\u03b21\/SMAD2 pathway is involved in angiogenesis during osteogenesis","volume":"21","author":"Ding A.","year":"2020","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-021-00649-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13073"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2343"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515598135"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.196"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22754-4"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24089"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12634"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202101412"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00015"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a008334"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.07.046"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25604"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2018.11.003"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034520924656"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034520937617"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27406"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.17133"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8090969"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2015.05.008"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03316-w"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.2337\/db17-1238"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3026"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.03.022"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2016.9"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2019.101504"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12794"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.340"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.910.BJR-2020-0140.R1"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3143"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abk0011"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.14736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T12:34:36Z","timestamp":1724416476000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.14736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,20]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1111\/odi.14736"],"URL":"https:\/\/doi.org\/10.1111\/odi.14736","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,20]]},"assertion":[{"value":"2023-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}