{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:54:02Z","timestamp":1772160842287,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:00:00Z","timestamp":1698364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170972"],"award-info":[{"award-number":["82170972"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this investigation was to examine the presence of interleukin (IL)\u201013 and its receptor IL\u201013R\u03b12 in the tissues of oral submucous fibrosis (OSF), investigate their biological functions, and explore the underlying mechanisms involved in the development of OSF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>The expression of IL\u201013 and IL\u201013R\u03b12 in the oral mucosa of patients with OSF and normal individuals was determined through immunohistochemistry and reverse transcription\u2013quantitative polymerase chain reaction (RT\u2013qPCR). Primary fibroblasts (FBs) were extracted through enzymatic digestion and then cultured. Immunofluorescence was employed to identify the FB cultures and the location of IL\u201013R\u03b12. The effects of IL\u201013\/IL\u201013R\u03b12\/PI3K\/AKT\/mTOR on the migration, proliferation, and secretion of fiber\u2010related proteins of FBs were explored via the wound healing assay, CCK\u20108 assay, EDU assay, and RT\u2013qPCR. The impact of IL\u201013R\u03b12 silencing and PI3K\/AKT inhibition on the effect of IL\u201013 on FBs was analyzed by RT\u2013qPCR and Western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IL\u201013 and IL\u201013R\u03b12 were highly expressed in OSF. Primary FBs were successfully extracted and cultured. IL\u201013R\u03b12 was found to be localized in myofibroblasts. IL\u201013 promoted the proliferation, migration, and secretion of fibril\u2010associated proteins in FBs. The proliferation, migration, and secretion of fibril\u2010associated proteins of FBs were decreased following IL\u201013R\u03b12 silencing and inhibition of the PI3K\/AKT\/mTOR pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>IL\u201013 may promote the proliferation, migration, and secretion of fiber\u2010related proteins of FBs through the PI3K\/AKT\/mTOR pathway by targeting IL\u201013R\u03b12.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14760","type":"journal-article","created":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T01:46:40Z","timestamp":1698457600000},"page":"3142-3154","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The mechanism of IL\u201013 targeting IL\u201013R\u03b12 in regulating oral mucosal FBs through 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