{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:20:35Z","timestamp":1791836435962,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:00:00Z","timestamp":1698019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Glucosyltransferases (<jats:italic>Gtfs<\/jats:italic>) and quorum sensing (QS) mediated transduction genes play critical roles in the pathogenesis of <jats:italic>Streptococcus mutan<\/jats:italic>\u2010mediated dental caries. Therefore, targeting <jats:italic>gtfs<\/jats:italic> and QS\u2010mediated virulence genes have therefore emerged as an intriguing goal for efficient therapeutic approaches that block cariogenic biofilms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Post\u2010biotic mediators (PMs) obtained from our previously isolated and characterized beneficial bacteria <jats:italic>Enterobacter colacae<\/jats:italic> PS\u201074 was assessed for its antibiofilm potential against <jats:italic>S. mutans<\/jats:italic>. According to the transcriptome method, qRT\u2010PCR analysis was performed against virulence genes. For microscopic visualization, SEM and CLSM analyses were used to confirm the inhibitory effects of PMs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PMs dramatically reduced the expression of QS signal transduction, glucan metabolism, and biofilm\u2010regulated genes such <jats:italic>gtfB<\/jats:italic>, <jats:italic>gtfC<\/jats:italic>, <jats:italic>ComDE<\/jats:italic>, <jats:italic>VicR<\/jats:italic>, <jats:italic>brpA<\/jats:italic> in <jats:italic>S. mutans<\/jats:italic>, which validates the outcomes of in\u00a0vitro result. Their unique metabolites may help to control biofilm formation by eluding antimicrobial resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Considering the above findings, PMs may deem to be an innovative, alluring, and secure method for preventing dental caries due to their biological activity. Our study unravels the inhibitory effect of PMs, which will contribute to instruct drug design strategies for effective inhibition of <jats:italic>S. mutans<\/jats:italic> biofilms.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.14780","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T09:54:36Z","timestamp":1698054876000},"page":"3471-3479","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Deciphering the mechanism of anti\u2010quorum sensing post\u2010biotic mediators against <i>Streptococcus mutans<\/i>"],"prefix":"10.1111","volume":"30","author":[{"given":"Preeti","family":"Pallavi","sequence":"first","affiliation":[{"name":"Centre for Biotechnology, School of Pharmaceutical Sciences Siksha \u2018O\u2019 Anusandhan (Deemed to be University)  Bhubaneswar 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