{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T21:42:16Z","timestamp":1785361336252,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T00:00:00Z","timestamp":1701648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to investigate the effect of plasma\u2010activated Ringer's lactate solution (PAL) on oral squamous cell carcinoma (OSCC) cells and carcinogenic processes with a particular focus on iron and collagenous matrix formation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      We used three OSCC cell lines, one keratinocyte cell line, and two fibroblast lines, and cell viability assays, immunoblotting, flow cytometry, and transmission electron microscopy were performed to evaluate the effect and type of cell death. The effect of PAL treatment on lysyl oxidase (LOX) expression was investigated in\u00a0vitro and in\u00a0vivo. Tamoxifen\u2010inducible\n                      <jats:italic>Mob1a\/b<\/jats:italic>\n                      double\u2010knockout mice were used for the in\u00a0vivo experiment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PAL killed OSCC cells more effectively than the control nontumorous cells and suppressed cell migration and invasion. Ferroptosis occurred and the protein level of LOX was downregulated in cancer cells in\u00a0vitro and in\u00a0vivo. Additionally, PAL improved the survival rate of mice and suppressed collagenous matrix formation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We demonstrated that PAL specifically kills OSCC cells and that ferroptosis occurs in\u00a0vitro and in\u00a0vivo. Furthermore, PAL can prevent carcinogenesis and improve the survival rate of oral cancer, especially tongue cancer, by changing collagenous matrix formation via LOX suppression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14827","type":"journal-article","created":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T09:44:30Z","timestamp":1701683070000},"page":"3912-3924","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Ferroptosis induced by plasma\u2010activated Ringer's lactate solution prevents oral cancer progression"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8442-5318","authenticated-orcid":false,"given":"Kotaro","family":"Sato","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Department of Pathology and Biological Responses Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Department of Pathology and Biological Responses Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kae","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Center for Low\u2010Temperature Plasma Sciences Nagoya University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromasa","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Center for Low\u2010Temperature Plasma Sciences Nagoya University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Hori","sequence":"additional","affiliation":[{"name":"Center for Low\u2010Temperature Plasma Sciences Nagoya University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Nishio","sequence":"additional","affiliation":[{"name":"Division of Molecular and Cellular Biology Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Division of Molecular and Cellular Biology Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideharu","family":"Hibi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Center for Low\u2010Temperature Plasma Sciences Nagoya University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5757-1109","authenticated-orcid":false,"given":"Shinya","family":"Toyokuni","sequence":"additional","affiliation":[{"name":"Department of Pathology and Biological Responses Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Center for Low\u2010Temperature Plasma Sciences Nagoya University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.202"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2022.105970"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3319"},{"key":"e_1_2_11_5_1","unstructured":"Cancer of the oral cavity and pharynx (invasive). 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