{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T17:47:48Z","timestamp":1778003268797,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173307"],"award-info":[{"award-number":["82173307"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872190"],"award-info":[{"award-number":["81872190"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7222225"],"award-info":[{"award-number":["7222225"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To explore the biological function and mechanisms of CEBPB and NAT10\u2010mediated N4\u2010acetylcytidine (ac4c) modification in salivary adenoid cystic carcinoma (SACC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>CEBPB and NAT10 were knocked down in SACC\u2013LM cells by siRNA transfection and overexpressed in SACC\u201083 cells by plasmid transfection. Malignant phenotypes were evaluated using CCK\u20108, Transwell migration and colony formation assays. Real\u2010time PCR, western blotting, ChIP and acRIP were used to investigate the molecular mechanisms involved.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that CEBPB was highly expressed in SACC tissues and correlated with lung metastasis and unfavourable prognosis. Gain\u2010 and loss\u2010of\u2010function experiments revealed that CEBPB promoted SACC malignant phenotypes. Mechanistically, CEBPB exerted its oncogenic effect by binding to the vimentin gene promoter region to enhance its expression. Moreover, NAT10\u2010mediated ac4c modification led to stabilization and overexpression of CEBPB in SACC cells. We also found that NAT10, the only known human enzyme responsible for ac4C modification, promoted SACC cell migration, proliferation and colony formation. Moreover, CEBPB overexpression restored the inhibitory effect of NAT10 knockdown on malignant phenotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study reveals the critical role of the newly identified NAT10\/CEBPB\/vimentin axis in SACC malignant progression, and the findings may be applied to improve treatment for SACC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.14879","type":"journal-article","created":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T05:23:50Z","timestamp":1706592230000},"page":"4341-4355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>NAT10<\/scp>\/<scp>CEBPB<\/scp>\/vimentin signalling axis promotes adenoid cystic carcinoma malignant phenotypes in\u00a0vitro"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5424-8441","authenticated-orcid":false,"given":"Min","family":"Fu","sequence":"first","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology  Beijing PR China"},{"name":"National Clinical Research Center for Oral Diseases  Beijing PR China"},{"name":"National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing PR China"},{"name":"Beijing Key Laboratory of Digital Stomatology  Beijing PR China"},{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mian","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Yi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng\u2010Lin","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology  Beijing PR China"},{"name":"National Clinical Research Center for Oral Diseases  Beijing PR China"},{"name":"National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing PR China"},{"name":"Beijing Key Laboratory of Digital Stomatology  Beijing PR China"},{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6012-2398","authenticated-orcid":false,"given":"Xi\u2010Yuan","family":"Ge","sequence":"additional","affiliation":[{"name":"Central Laboratory Peking University School and Hospital of Stomatology  Beijing PR China"},{"name":"National Clinical Research Center for Oral Diseases  Beijing PR China"},{"name":"National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing PR China"},{"name":"Beijing Key Laboratory of Digital Stomatology  Beijing PR China"},{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"issue":"1872","key":"e_1_2_9_2_1","article-title":"Acetylation of cytidine in mRNA promotes translation efficiency","volume":"175","author":"Arango D.","year":"2018","journal-title":"Cell"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2020.01.023"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-020-0253-2"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac404"},{"key":"e_1_2_9_6_1","first-page":"197","article-title":"Adenoid cystic carcinoma","volume":"84","author":"Castelnuovo P.","year":"2020","journal-title":"Adv Otorhinolaryngol"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-021-01988-y"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.27408"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-022-01338-9"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2020.5069"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-022-00197-x"},{"key":"e_1_2_9_12_1","first-page":"1","article-title":"PTPN14 promotes gastric cancer progression by PI3KA\/AKT\/mTOR pathway","volume":"14","author":"Li H.","year":"2023","journal-title":"Cell Death & Disease"},{"issue":"29","key":"e_1_2_9_13_1","first-page":"62","article-title":"Stablishment of a human cancer cell line from adenoid cystic carcinoma of the minor salivary gland","volume":"25","author":"Li S.","year":"1990","journal-title":"Zhonghua Kou Qiang Yi Xue Za Zhi"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-023-00793-4"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.861000"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-023-10543-9"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1111522"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2022.10.004"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.12.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddad022"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2418-2"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-022-00553-x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-020-09444-5"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12010184"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-023-02275-1"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115477"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.738"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202201244RRRR"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24703"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2119038119"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2023.03.023"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-021-00888-8"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-023-02628-3"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12363"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-022-03467-8"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.14879","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T07:09:12Z","timestamp":1737011352000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.14879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/odi.14879"],"URL":"https:\/\/doi.org\/10.1111\/odi.14879","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,29]]},"assertion":[{"value":"2023-10-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}