{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:27:24Z","timestamp":1791638844883,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:00:00Z","timestamp":1709164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Ameloblastoma (AM), a locally aggressive tumor with extensive growth capacity, causes significant damage to the jaw and affects facial appearance. Although the high prevalence of <jats:italic>BRAF<\/jats:italic> V600E mutation in AM is known, its specific impacts on patients with AM remain unclear. Thus, the present study investigated the role of <jats:italic>BRAF<\/jats:italic> V600E mutation, thereby focusing on its impact on AM invasion and growth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Immunohistochemical analysis was used to compare BRAF V600E, MMP2, MMP9, and Ki\u201067 expressions in AM (<jats:italic>n<\/jats:italic>\u2009=\u200949), normal oral mucosa (NOM) (<jats:italic>n<\/jats:italic>\u2009=\u200910), and odontogenic keratocyst (OKC) (<jats:italic>n<\/jats:italic>\u2009=\u200915) tissues. AM was further classified according to the presence or absence of BRAF V600E. The relationship between BRAF V600E and invasion as well as growth was evaluated. In addition, correlation analysis was performed using immunohistochemistry and confirmed via double\u2010labeling immunofluorescence. Finally, comparative analyses using mass spectrometry, immunohistochemistry, and immunofluorescence were performed to explore and identify underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>AM exhibited a higher incidence of <jats:italic>BRAF<\/jats:italic> V600E mutation than NOM and OKC. BRAF V600E expression was positively correlated with the invasion\u2010associated proteins MMP2 and MMP9 and the growth\u2010related protein Ki\u201067. Proteomic data revealed that <jats:italic>BRAF<\/jats:italic> V600E primarily activates the MAPK signaling pathway in AM, particularly driving the phosphorylation of extracellular signal\u2010regulated kinase 1\/2 (ERK1\/2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In summary, the findings suggested that the <jats:italic>BRAF<\/jats:italic> V600E mutation enhances the invasion and growth abilities of AM via the MAPK\/ERK signaling pathway. Thus, targeting <jats:italic>BRAF<\/jats:italic> V600E or the MAPK\/ERK pathway may be a potential AM therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.14909","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T05:49:54Z","timestamp":1709272194000},"page":"4426-4439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>BRAF<\/i><scp>V600E<\/scp> mutation mediates invasive and growth features in ameloblastoma"],"prefix":"10.1111","volume":"30","author":[{"given":"Chen\u2010Xi","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin\u2010Zhou","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8478-2086","authenticated-orcid":false,"given":"Qi\u2010Wen","family":"Man","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Oral Maxillofacial Head Neck Oncology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2103-5494","authenticated-orcid":false,"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Oral Maxillofacial Head Neck Oncology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.ccr\u201014\u20101069"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1124\/pharmrev.121.000349"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9010198"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784\u2010018\u20102494\u2010y"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032\u2010023\u201001993\u2010z"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.31557\/apjcp.2023.24.1.267"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12646"},{"key":"e_1_2_8_9_1","unstructured":"EI\u2010Naggar A. 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