{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T21:45:07Z","timestamp":1774907107631,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Neutrophil response is critical in inflammatory regulation and immune response to bacterial infections. During periodontal disease, pathogenic bacteria lead to exaggerated neutrophil responses. We hypothesized that low\u2010level laser application (LLLT), therapeutic strategy for dampening inflammatory processes, will regulate neutrophil activity in response to periodontopathogens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      The impact of LLLT on neutrophil responses was measured by light delivered at wavelength of 850\u2009nm. The direct effect of LLLT on\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      A7436 was determined by flow cytometry using LIVE\/DEADTM Cell Vitality kit. The phagocytosis of\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      A7436 by human neutrophils was measured using flow cytometry. Superoxide generation was measured by cytochrome\u2010C\u2010reduction in the presence of N\u2010formyl\u2010L\u2010methionyl\u2010L\u2010leucyl\u2010L\u2010phenylalanine (fMLP; 1\u2009mM). Cytokine release by neutrophils was measured by multiplex immunoassay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The phagocytosis of\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      by primary human neutrophils was significantly reduced in response to LLLT (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). While LLLT led to increased superoxide production in neutrophils that were not challenged by\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      , it dampened the increased superoxide and IL\u20106 release by the neutrophils in response to\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      . LLLT did not directly affect the viability of\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These results suggested that LLLT can provide therapeutic strategy in periodontal disease, regulating the neutrophil response to\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14954","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T08:01:47Z","timestamp":1712649707000},"page":"5268-5273","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Neutrophil response to\n                    <i>Porphyromonas gingivalis<\/i>\n                    is modulated by low\u2010level laser application"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7333-0524","authenticated-orcid":false,"given":"Yerda","family":"\u00d6zkan Karasu","sequence":"first","affiliation":[{"name":"ADA Forsyth Institute  Cambridge Massachusetts USA"},{"name":"Faculty of Dentistry, Department of Periodontology Ataturk University  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatma","family":"\u00d6ner","sequence":"additional","affiliation":[{"name":"ADA Forsyth Institute  Cambridge Massachusetts USA"},{"name":"Faculty of Dentistry, Department of Periodontology Bahcesehir University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2679-9100","authenticated-orcid":false,"given":"Alpdogan","family":"Kantarci","sequence":"additional","affiliation":[{"name":"ADA Forsyth Institute  Cambridge Massachusetts USA"},{"name":"School of Dental Medicine Harvard University  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-007-6211-6"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2007.2119"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/1066896907302239"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1984.tb01012.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2005.00783.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201600035"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-011-0454-7"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1999.tb02234.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.6.2097-2104.1991"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-018-2569-7"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-015-1724-7"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02461.x"},{"key":"e_1_2_10_14_1","first-page":"883","article-title":"Photobiomodulation with 670 nm light increased phagocytosis in human retinal pigment epithelial cells","volume":"21","author":"Fuma S.","year":"2015","journal-title":"Molecular Vision"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.02950.x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01113.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00883.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3785"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/1011-1344(95)07239-X"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1995.tb08631.x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00053"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000136335"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-014-1571-y"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rdc.2021.04.002"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.10126"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2006.00204.x"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-010-0850-5"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2009.09.002"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020076"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00189.x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1982.tb01182.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9pp00089e"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1800045"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13112"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13060901"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-016-0371-x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.4.1391-1396.2005"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201300015"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201400064"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411900010020201"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2016.10.008"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apcsb.2019.12.001"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-019-02873-7"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.14954","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T09:43:13Z","timestamp":1733132593000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.14954"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,9]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1111\/odi.14954"],"URL":"https:\/\/doi.org\/10.1111\/odi.14954","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/ODI.14954\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14954\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14954\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14954\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/ODI.14954\/v2\/decision1","asserted-by":"object"}]},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,9]]},"assertion":[{"value":"2024-02-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}