{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:13:01Z","timestamp":1792109581804,"version":"4.4.0"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aimed to assess the effects of\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      outer membrane vesicles (\n                      <jats:italic>Pg<\/jats:italic>\n                      \u2010OMVs) in chronic periodontitis and explore the underlying mechanism involved.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In vitro, Pg\u2010OMVs were incubated with Ea.hy926 (vessel endothelial cells, ECs) to evaluate their effects on endothelial functions and to investigate the underlying mechanism. The effects of endothelial dysfunction on MG63 osteoblast\u2010like cells were verified using an indirect co\u2010culture method. For in\u00a0vivo studies, micro\u2010CT was conducted to identify alveolar bone mass. Immunofluorescence staining was conducted to confirm the levels of stimulator of interferon genes (STING) in the blood vessel and the number of Runx2\n                      <jats:sup>+<\/jats:sup>\n                      cells around the alveolar bone.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pg<\/jats:italic>\n                      \u2010OMVs were endocytosed by ECs, leading to endothelial dysfunction. The cGAS\u2010STING\u2010TBK1 pathway was activated in ECs, which subsequently inhibited MG63 migration and early osteogenesis differentiation. In\u00a0vivo,\n                      <jats:italic>Pg<\/jats:italic>\n                      \u2010OMVs promoted alveolar bone resorption, increased STING levels in the blood vessel, and decreased Runx2\n                      <jats:sup>+<\/jats:sup>\n                      cells around the alveolar bone.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pg<\/jats:italic>\n                      \u2010OMVs caused endothelial dysfunction and activated the cGAS\u2010STING\u2010TBK1 signal cascade in ECs, thereby impairing ECs\u2010mediated osteogenesis. Furthermore,\n                      <jats:italic>Pg<\/jats:italic>\n                      \u2010OMVs aggregated alveolar bone loss and altered the blood vessel\u2010mediated osteogenesis with elevated STING.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14969","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T13:40:17Z","timestamp":1714657217000},"page":"5461-5474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<i>Porphyromonas gingivalis<\/i>\n                    <scp>OMVs<\/scp>\n                    promoting endothelial dysfunction via the\n                    <scp>STING<\/scp>\n                    pathway in periodontitis"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1154-4142","authenticated-orcid":false,"given":"Zhenwei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of 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