{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T08:20:04Z","timestamp":1777537204555,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204421"],"award-info":[{"award-number":["82204421"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to clarify the relationship between FADD amplification and overexpression and the tumor immune microenvironment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Immunohistochemical staining and bioanalysis were used to analyze the association between FADD expression in tumor cells and cells in tumor microenvironment. RNA\u2010seq analysis was used to detect the differences in gene expression upon FADD overexpression. Flow cytometry and multicolor immunofluorescence staining (mIHC) were used to detect the differences in CD8\n                      <jats:sup>+<\/jats:sup>\n                      T\u2010cell infiltration in FADD\u2010overexpressed cells or tumor tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overexpression of FADD significantly promoted tumor growth. Cells with high FADD expression presented high expression of CD276 and FGFBP1 and low expression of proinflammatory factors (such as IFIT1\u20103 and CXCL8), which reduced the percentage of CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells and created a \u201ccold tumor\u201d immune microenvironment, thus promoting tumor progression. In\u00a0vivo and in\u00a0vitro experiment confirmed that tumor tissues with excessive FADD expression exhibited CD8\n                      <jats:sup>+<\/jats:sup>\n                      T\u2010cell exclusion in the microenvironment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our preliminary investigation has discovered the association between FADD expression and the immunosuppressive microenvironment in HNSCC. Due to the high frequent amplification of the chromosomal region 11q13.3, where FADD is located, targeting FADD holds promise for improving the immune\u2010inactive state of tumors, subsequently inhibiting HNSCC tumor progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.14976","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T13:20:27Z","timestamp":1714656027000},"page":"5007-5021","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>FADD<\/scp>\n                    amplification is associated with\n                    <scp>CD8<\/scp>\n                    <sup>+<\/sup>\n                    T\u2010cell exclusion and malignant progression in\n                    <scp>HNSCC<\/scp>"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2334-0731","authenticated-orcid":false,"given":"Yang","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head Neck Oncology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine Chinese Academy of Medical Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2677-8104","authenticated-orcid":false,"given":"Surui","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head Neck Oncology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine Chinese Academy of Medical Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanni","family":"Ma","sequence":"additional","affiliation":[{"name":"Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinan","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruixin","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head Neck Oncology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine Chinese Academy of Medical Sciences  Shanghai 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