{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T19:07:46Z","timestamp":1793300866770,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["11702154"],"award-info":[{"award-number":["11702154"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171303"],"award-info":[{"award-number":["32171303"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Insufficient occlusal support (IOS) frequently causes subchondral bone absorption in temporomandibular joint osteoarthritis, and the underlying mechanism requires further investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An IOS model was established by abrading rat molars. Micro\u2010computed tomography was used to evaluate subchondral bone changes. Osteoclastogenesis of synovium\u2010derived macrophages (SDMs) was confirmed by TRAP staining. Cartilage\u2010specific TNF\u03b1 depletion was achieved by intra\u2010articular injection of adeno\u2010associated virus carrying shRNA against murine TNF\u03b1 under control of collagen type II. In\u00a0vitro, chondrocytes were mechanically compressed and conditioned medium (CM) was collected to detect its ability to induce osteoclastogenesis of SDMs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Synovial osteoclastogenesis and condyle resorption were observed following IOS. TNF\u03b1 level was elevated in hypertrophic chondrocytes after IOS. Synovial Wnt5a level increased, but Wnt3a level decreased after IOS. Depletion of TNF\u03b1 in chondrocytes alleviated the synovial osteoclastogenesis and condyle bone resorption. In\u00a0vitro compression of chondrocytes potentiated TNF\u03b1 expression and secretion. The CM promoted osteoclastogenesis of SDMs, which were partially prohibited by TNF\u03b1 neutralizing antibody. Furthermore, inhibition of Wnt3a facilitated osteoclastogenesis, whereas inhibition of Wnt5a partially suppressed osteoclastogenesis, of SDMs cultured in CM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Chondrocyte\u2010secreted TNF\u03b1 induced by IOS is a critical regulator of synovial osteoclastogenesis and subsequent condylar resorption, partially through non\u2010canonical Wnt5a pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.14978","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T06:45:43Z","timestamp":1715237143000},"page":"5140-5153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Synovial osteoclastogenesis mediated by chondrocyte\u2010secreted <scp>TNF\u03b1<\/scp> promotes <scp>TMJ<\/scp> condylar resorption"],"prefix":"10.1111","volume":"30","author":[{"given":"Yan","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"},{"name":"Department of Central Laboratory The Affiliated Hospital of Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Ran","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"},{"name":"Department of Central Laboratory The Affiliated Hospital of Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeng","family":"Xuemin","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  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