{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:53:59Z","timestamp":1772160839816,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2024,11]]},"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n \n Our previous studies have found that the composition ratio of\n Prevotella melaninogenica<\/jats:italic>\n (\n Pm<\/jats:italic>\n ) on buccal mucosa surface of oral lichen planus (OLP) patients increased significantly compared with control. Furthermore,\n Pm<\/jats:italic>\n could invade the epithelium of OLP patients. This study aimed to further explore the impact of\n Pm<\/jats:italic>\n on oral keratinocytes.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n The\n Pm<\/jats:italic>\n \u2013human oral keratinocyte (HOK) co\u2010culture model was established to detect monolayer permeability, zona occludens\u20101 (ZO\u20101) expression, and intracellular survival of\n Pm<\/jats:italic>\n . We performed RNA\u2010seq followed by identification of differentially expressed genes (DEGs) and Gene Ontology (GO) analysis, with a particular focus on myosin light chain kinase (MLCK). An MLCK inhibitor ML\u20107 was utilized in\n Pm<\/jats:italic>\n \u2010HOK co\u2010culture model to assess its effects on monolayer permeability and ZO\u20101 expression.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n HOK monolayer permeability was increased, and ZO\u20101 expression was decreased after co\u2010culture (\n p<\/jats:italic>\n \u2009<\u20090.05).\n Pm<\/jats:italic>\n could survive in HOK cells. RNA\u2010seq revealed MLCK was an upregulated common DEG. The expression of MLCK in the\n Pm<\/jats:italic>\n \u2010HOK co\u2010culture model was upregulated. Inhibition of MLCK rescued the increased epithelial permeability, and ZO\u20101 expression was upregulated (\n p<\/jats:italic>\n \u2009<\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n MLCK may be involved in disrupting epithelial barrier function by\n Pm<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/odi.14980","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T02:29:26Z","timestamp":1715221766000},"page":"5102-5112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prevotella melaninogenica<\/i>\n disrupted oral epithelial barrier function via myosin light chain kinase"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2222-2232","authenticated-orcid":false,"given":"Yiting","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Oral Medicine Stomatology Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Shanghai 10th People's Hospital & School of Life Sciences and Technology Tongji University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9809-9472","authenticated-orcid":false,"given":"Yuan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Stomatology Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai 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