{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:13:04Z","timestamp":1792109584152,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T00:00:00Z","timestamp":1721952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study investigated the role of fibrin on neutrophil extracellular traps (NETs) formation from neutrophils and to elucidate the involvement of mitochondria in NETs formation during periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Plasminogen\u2010deficient (\n                      <jats:italic>Plg<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      ) mice were employed to evaluate the effects of fibrin deposition on inflammation, bone resorption, and neutrophil infiltration in periodontal tissues. In addition, in\u00a0vitro tests evaluated fibrin's impact on neutrophil\u2010driven inflammation. Mitochondrial reactive oxygen species (mtROS) levels within neutrophils were quantified utilizing flow cytometry and immunofluorescence in\u00a0vitro. Furthermore, the anti\u2010inflammatory properties of the mtROS scavenger, Mito\u2010TEMPO, were confirmed to regulate the NET formation in\u00a0vitro and in\u00a0vivo.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Plasminogen deficiency resulted in increased fibrin deposition, neutrophil infiltration, inflammatory factors concentration, and alveolar bone resorption in periodontal tissues. After neutrophils were treated by fibrin in\u00a0vitro, the expression of inflammatory factors, the formation of mtROS, and NETs enriched in mitochondrial DNA (mtDNA) were upregulated, which were reversed by Mito\u2010TEMPO in\u00a0vitro. Moreover, Mito\u2010TEMPO alleviated inflammation in\n                      <jats:italic>Plg<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study showed that fibrin deposition in gingiva induced the NET formation in\n                      <jats:italic>Plg<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice, in which the DNA in NETs was from mitochondria depending on increasing mtROS.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15073","type":"journal-article","created":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T03:19:07Z","timestamp":1721963947000},"page":"577-588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Fibrin aggravates periodontitis through inducing\n                    <scp>NETs<\/scp>\n                    formation from mitochondrial\n                    <scp>DNA<\/scp>"],"prefix":"10.1111","volume":"31","author":[{"given":"Yinan","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry Shanghai Stomatological Hospital, Fudan University  Shanghai China"},{"name":"Shanghai Medical College Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University  Shanghai 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