{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:19:12Z","timestamp":1773101952211,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,8,18]],"date-time":"2024-08-18T00:00:00Z","timestamp":1723939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E\u201026\/201.289\/2022"],"award-info":[{"award-number":["E\u201026\/201.289\/2022"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E\u201026\/200.331\/2024"],"award-info":[{"award-number":["E\u201026\/200.331\/2024"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To investigate\n                      <jats:italic>Treponema pallidum<\/jats:italic>\n                      detection using immunohistochemistry (IHC) and reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) assays in acquired oral syphilis (AOS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Thirty\u2010seven paraffin\u2010embedded tissue specimens of AOS (32 secondary and five primary) were analyzed, integrating double\u2010positive serological results with clinicodemographic and histopathological data.\n                      <jats:italic>T.\u2009pallidum<\/jats:italic>\n                      presence was semiquantitatively assessed by IHC, while RT\u2010PCR targeted\n                      <jats:italic>T.\u2009pallidum<\/jats:italic>\n                      DNA. Sensitivity, specificity, and the area under the curve (AUC) were calculated with 95% confidence intervals (CI).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The study included mostly females (62.2%) with a mean age of 27.1\u2009years.\n                      <jats:italic>T.\u2009pallidum<\/jats:italic>\n                      was detected in all samples by IHC, predominantly in the epithelium across all layers (43.2%). RT\u2010PCR identified\n                      <jats:italic>T.\u2009pallidum<\/jats:italic>\n                      DNA in 32 cases, with negative results observed in cases of secondary AOS. The AUC for IHC versus disease stage was 62.5% (95% CI: 45.1\u201377.8), and for RT\u2010PCR, it was 57.8% (95% CI: 40.5\u201373.8). The AUC comparing IHC to RT\u2010PCR was 83.8% (95% CI: 67.9\u201393.8).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study represents the first attempt to evaluate the proposed direct detection algorithm for AOS. IHC and RT\u2010PCR serve as ancillary tools for detecting\n                      <jats:italic>T.\u2009pallidum<\/jats:italic>\n                      in both primary and secondary stages of AOS.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15107","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T01:04:06Z","timestamp":1724029446000},"page":"890-898","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Immunohistochemistry and\n                    <scp>RT<\/scp>\n                    \u2010\n                    <scp>PCR<\/scp>\n                    as ancillary tools in the diagnosis of acquired oral syphilis"],"prefix":"10.1111","volume":"31","author":[{"given":"Maria Eduarda Zeraik","family":"Barreto","sequence":"first","affiliation":[{"name":"Department of Oral Diagnosis and Pathology School of Dentistry, Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6861-6640","authenticated-orcid":false,"given":"Ciro Dantas","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Pathology Getulio Sales Diagn\u00f3sticos  Natal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u00edra Medeiros Pacheco","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Department of Pathology Getulio Sales Diagn\u00f3sticos  Natal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre","family":"de Oliveira Sales","sequence":"additional","affiliation":[{"name":"Department of Pathology Getulio Sales Diagn\u00f3sticos  Natal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6657-4547","authenticated-orcid":false,"given":"Felipe Paiva","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Pathology and Clinical Dentistry, School of Dentistry, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2258-8071","authenticated-orcid":false,"given":"Lucas Guimar\u00e3es","family":"Abreu","sequence":"additional","affiliation":[{"name":"Department of Child and Adolescent Oral Health School of Dentistry, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6986-9148","authenticated-orcid":false,"given":"Jefferson R.","family":"Ten\u00f3rio","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis and Pathology School of Dentistry, Universidade Federal do Rio de Janeiro  Rio de Janeiro 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