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Protein identification and quantification were performed using MaxQuant software. Statistical analysis and functional enrichment were conducted using the Perseus platform and STRING tool, respectively. Immunohistochemistry was used for validation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Comparative proteomic analysis revealed 2680 proteins across the three tissue types, with 799 significantly altered between groups. Translocation protein SEC63 homolog, Annexin A6 and Biglycan were up\u2010regulated in CXPA compared to PA. Decorin was markedly up\u2010regulated in both PA and CXPA compared to normal salivary gland (log\n                      <jats:sub>2<\/jats:sub>\n                      fold changes of 7.58 and 7.38, respectively). Validation confirmed elevated levels of Biglycan and Decorin in the extracellular matrix of CXPA compared to PA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Proteomic analysis identified differential protein expression patterns associated with malignant transformation of PA into CXPA. Findings indicate a crucial role for extracellular matrix proteins, specifically Biglycan and Decorin, in the tumorigenic progression of PA and CXPA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15109","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T01:39:48Z","timestamp":1724031588000},"page":"865-878","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pleomorphic adenoma and carcinoma ex\u2010pleomorphic adenoma tumorigenesis: A proteomic analysis"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5721-9968","authenticated-orcid":false,"given":"Virg\u00edlio Gonzales","family":"Zanella","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Dentistry Federal University of Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Head and Neck Surgery Department Santa Rita Hospital, Santa Casa de Miseric\u00f3rdia de Porto Alegre  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5150-9227","authenticated-orcid":false,"given":"Sara Ferreira Dos Santos","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology, School of Dentistry Federal University of Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0993-936X","authenticated-orcid":false,"given":"Lauren Frenzel","family":"Schuch","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School University of Campinas  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3417-3586","authenticated-orcid":false,"given":"Emily Ferreira Salles","family":"Pilar","sequence":"additional","affiliation":[{"name":"Experimental Research Unit Hospital de Cl\u00ednicas Porto Alegre  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7959-147X","authenticated-orcid":false,"given":"Adriana Franco","family":"Paes Leme","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School University of Campinas  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7225-5879","authenticated-orcid":false,"given":"Jean Nunes","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Postgraduation Program in Dentistry and Health Federal University of Bahia  Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0378-9380","authenticated-orcid":false,"given":"Syed Ali","family":"Khurram","sequence":"additional","affiliation":[{"name":"Academic Unit of Oral and Maxillofacial Medicine and Pathology, Department of 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