{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:53:57Z","timestamp":1772160837935,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T00:00:00Z","timestamp":1729123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","award":["202140357"],"award-info":[{"award-number":["202140357"]}],"id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008750","name":"Shanghai Municipal Hospital Development Center","doi-asserted-by":"publisher","award":["SHDC12023115"],"award-info":[{"award-number":["SHDC12023115"]}],"id":[{"id":"10.13039\/501100008750","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study investigated the role of autophagy on osteogenesis of DFCs under inflammatory microenvironment during tooth eruption.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>DFCs were isolated and identified. Lipopolysaccharide (LPS) was used to construct the inflammatory microenvironment in\u00a0vitro and in\u00a0vivo. Cell viability was examined by CCK\u20108 assay. Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining, alizarin red S (ARS) staining. The gene and protein levels were examined using qRT\u2010PCR and western blot analysis, respectively. We observed the process of tooth eruption after local LPS injection by micro\u2010CT and HE staining. Osteogenesis and autophagy were monitored through qRT\u2010PCR, western blot and histological staining of specific markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>LPS at the indicated concentrations did not produce toxic effects on DFCs, and significantly promoted the inflammatory gene expression. LPS inhibited osteogenic differentiation and activated autophagy in DFCs. Blocking autophagy with 3\u2010MA reversed the expression of osteogenic markers in LPS\u2010treated DFCs. Additionally, the eruption of LPS\u2010treated teeth was accelerated and their DFs exhibited an increased expression of TNF\u2010\u03b1 and Beclin1, and decreased expression of ALP and RUNX2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Autophagy was involved in the suppression of the DFCs osteogenesis in an LPS\u2010induced inflammatory condition, suggesting the pivotal role of autophagy in inflammation\u2010induced premature tooth eruption.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15149","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T03:12:49Z","timestamp":1729134769000},"page":"928-940","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Role of Autophagy on Osteogenesis of Dental Follicle Cells Under Inflammatory Microenvironment"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4496-2642","authenticated-orcid":false,"given":"Zhen","family":"Jiang","sequence":"first","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Pediatric Dentistry, Stomatological Hospital and Dental School Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Pediatric Dentistry, Stomatological Hospital and Dental School Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linjuan","family":"Luo","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Pediatric Dentistry, Stomatological Hospital and Dental School Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0135-5917","authenticated-orcid":false,"given":"Beizhan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Pediatric Dentistry, Stomatological Hospital and Dental School Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,17]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12549"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11070997"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.591803"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12010138"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/ccd.ccd_68_20"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.17796\/jcpd.29.3.5238p10v21r2j162"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-021-03998-2"},{"issue":"1","key":"e_1_2_14_9_1","first-page":"664","article-title":"Substance P Enhances BMSC Osteogenic Differentiation via Autophagic Activation","volume":"20","author":"Geng W.","year":"2019","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/1807-3107bor-2018.vol32.0069"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623310396903"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050062"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.892610"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.21-0178"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1797280"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.job.2022.02.005"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2020.01.009"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10266-019-00461-3"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e15854"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.02.062"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/dj9010007"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-042617-053253"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2020.0118"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2020.07.006"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23115945"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.07.016"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2012.10.024"},{"issue":"9","key":"e_1_2_14_28_1","article-title":"Effect of Chronic Apical Periodontitis in Primary Molars on Succedaneous Permanent Teeth: An Observational Retrospective Study","volume":"15","author":"Patil N.","year":"2023","journal-title":"Cureus"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514553815"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.397"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1032-3"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2020.104951"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.08.029"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-016-9690-4"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00082-12"},{"issue":"11","key":"e_1_2_14_36_1","first-page":"880","article-title":"Lipopolysaccharide (LPS)\u2010induced Autophagy is Responsible for Enhanced Osteoclastogenesis","volume":"40","author":"Sul O. 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