{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T02:08:24Z","timestamp":1779934104744,"version":"3.53.1"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007683","name":"Nihon University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007683","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007854","name":"School of Dentistry, Nihon University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007854","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Although macrophage polarisation in the trigeminal ganglion (TG) is crucial in orofacial pain hypersensitivity, the effect of ageing\u2010related changes and their involvement in intra\u2010oral nociception remains unclear. We assessed the effect of ageing\u2010related macrophage polarisation in TG on intra\u2010oral mechanical pain hypersensitivity following palatal mucosal incision using senescence\u2010accelerated mice (SAM)\u2010prone8 (SAMP8) and SAM\u2010resistant 1 (SAMR1).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Mechanical head\u2010withdrawal reflex threshold (MHWRT) of the palatal mucosa was measured for 21\u2009days after palatal mucosal incision. On days 3 and 14, the abundance of Iba\u20101\u2010immunoreactive (IR) cells, CD11c\u2010IR cells (pro\u2010inflammatory macrophages (M1)), C\u2010C motif chemokine ligand 2 (CCL2)\u2010IR M1\u2010macrophages, CD206\u2010IR cells (anti\u2010inflammatory macrophages (M2)) and transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2)\u2010IR M2\u2010macrophages in the TG was analysed. The effect of continuous intra\u2010TG administration of CCL2\u2010neutralising antibody or recombinant\u2010CCL2 on MHWRT was examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Incision\u2010induced decrease in MHWRT was enhanced in SAMP8 compared with that in SAMR1. On days 3 and 14, the number of CCL2\u2010IR M1\u2010macrophages in TG was increased in SAMP8 compared with that in SAMR1. CCL2\u2010neutralising antibody suppressed, whereas recombinant\u2010CCL2 increased pain hypersensitivity in SAMP8.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Mechanical pain hypersensitivity after oral mucosal injury is potentiated and sustained by age\u2010related enhancement of CCL2 signalling via M1\u2010macrophage hyperactivation in TG.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.15165","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T14:32:11Z","timestamp":1730125931000},"page":"600-610","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ageing\u2010Related Macrophage Polarisation in the Trigeminal Ganglion Enhances Incisional Intraoral Pain"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6034-5305","authenticated-orcid":false,"given":"Kentaro","family":"Urata","sequence":"first","affiliation":[{"name":"Department of Complete Denture Prosthodontics Nihon University School of Dentistry  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuki","family":"Oto","sequence":"additional","affiliation":[{"name":"Department of Complete Denture Prosthodontics Nihon University School of Dentistry  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Physiology Nihon\u00a0University School of Dentistry  Chiyoda\u2010ku Tokyo 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